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A study on relative quantification method of detecting ruminal fibrolytic bacteria of dairy cattle by real-time fluorescent quantitative polymerase chain reaction



A study on relative quantification method of detecting ruminal fibrolytic bacteria of dairy cattle by real-time fluorescent quantitative polymerase chain reaction



China Dairy Cattle: 10-14



Three healthy Chinese Holstein lactating cows with permanent ruminal cannulas were involved to select the validation of a real-time fluorescent quantitative PCR. Three methods (SCF, David M.Stevenson and Pfaffl, M.W.) for the real-time fluorescent quantitative PCR were compared. Based on the relative quantitative results for the gene of the same strain, significant correlation was found between the methods of SCF and Pfaffl, M.W. (<i>r</i> = 0.96-0.99, <i>P</i><0.05).

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