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Acute effect of a single in vivo injection of cortisol on in vitro amino acid incorporating activity of rat liver and thymic preparations



Acute effect of a single in vivo injection of cortisol on in vitro amino acid incorporating activity of rat liver and thymic preparations



Biochemical and Biophysical Research Communications 16(5): 449-454



It is evident that both liver and thymic tissue taken from rats 3 hours following a single injection of cortisol show a response to the hormone, but in opposite ways with regard to hormonal influence on cell free amino acid incor-porating systems prepared from the 2 tissues. The stimulating and inhibiting effects, respectively, on liver and thymic preparations are evident from the data. No significant change in thymic weight was evident at the time of sacrifice of the animals. Preliminary histologic studies also indicate no significant influence on thymus morphology of this dose of cortisol given 3 hours prior to removal of the tissue. From initial studies designed to localize the hormonal effect, the following observations seem pertinent. The stimulatory effect of cortisol in liver resides in the microsomal fraction. The inhibitory influence of the thymic preparations is evident both in the 105,000 x g supernatant solutions and in the sedimented microsomal fractions of homogenates of thymus from cortisol-injected rats. Moreover, such high speed supernatant solutions show a greater inhibitory effect than does the control thymic cell sap on the leucine incorporating activity of liver microsomal preparations from either uninjected or cortisol injected rats. Experiments are in progress to examine the locus of the observed cortisol influence in thymic tissue and to assess further the previous suggestions of the nature of adrenal cortical steroid action in liver protein synthesis.

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Accession: 029888503

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PMID: 5871832


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