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An automated fluorometric assay for alkaline phosphatase using 3-O-methylfluorescein phosphate

An automated fluorometric assay for alkaline phosphatase using 3-O-methylfluorescein phosphate

Analytical Biochemistry 24(1): 9-17

Refrigeration of the substrate 3-O-methylfluorescein phosphate and attention to cleanliness of the glassware conferred sufficient stability to allow development of an automated [AutoAnalyzer] fluorometric procedure for measurement of alkaline phosphatase activity at rates of up to 60 samples per hour over the range 0.2-5.6 pico-moles/ml of 3-O-methylfluorescein liberated per minute incubation at 37CC. Optimum reagent concentrations were determined which produced linear relationships between fluorescence produced by increasing concentrations of enzyme and by increasing time of incubation.

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Accession: 030012781

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PMID: 5665203

DOI: 10.1016/0003-2697(68)90054-7

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