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An evaluation of cresyl echt violet acetate as a Nissl stain


An evaluation of cresyl echt violet acetate as a Nissl stain



Stain Technology 30(2): 83-88



ISSN/ISBN: 0038-9153

PMID: 14358982

DOI: 10.3109/10520295509113749

The new cresyl echt violet acetate, of which 3 different batches have been tested, proves to by a very useful Nissl stain. It is especially valuable for formalin-fixed, frozen-sectioned material. By using a buffered staining bath and controlled timing in dehydration it is possible, on paraffin embedded material, to use these dyes as progressive stains apparently specific for nucleic acids. With a saturated aqueous solution of the dye, especially when a mordant of lithium carbonate is used, it is possible to stain material that has been preserved in formalin for several years and also material from which nucleic acids have been removed. The dye is useful also for staining celloidin embedded material. With the buffered stain proposed, differentiation is much easier than with older methods which included a gross overstaining and a long distaining procedure.

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Accession: 030020768

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