+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

An evaluation of random amplified polymorphic DNA for the identification and phylogeny of freshwater snails of the genus Bulinus



An evaluation of random amplified polymorphic DNA for the identification and phylogeny of freshwater snails of the genus Bulinus



Journal of Molluscan Studies 62(2): 165-176



The Random Amplified Polymorphic DNA (RAPD) assay was used to study genetic variation within and between 9 species of the genus Bulinus and to determine whether RAPD profiles could be used as markers for identification purposes. RAPDs were generated with 8 primers of two different sizes (10mers and 15mers) and were visualised using both polyacrylamide gel electrophoresis (PAGE) with silver staining and agarose gel electrophoresis with ethidium bromide staining. The species groups of Bulinus had few similarities in their RAPD profiles and there was interspecific variation within groups. Intrapopulation variation was observed, with all primers, for B. globosus collected from a single site in Zimbabwe. PAGE/silver staining methods visualised a greater number of RAPDs in comparison with agarose/ethidium bromide methods. Phenetic analysis indicated that distance estimates between taxa were sometimes non-additive and the phylogenetic analysis of such non-metric data is discussed. The resultant phenograms, constructed using a least squares method, were constrained almost into a polytomy with topologies often differing between data sets. It was concluded that this phenomenon was most likely attributable to large nucleotide divergences between the species groups which go beyond the phylogenetic scope of RAPD analysis. RAPD profiles, when used in conjunction with other taxonomic methods, may contribute to the identification of species of Bulinus on a regional basis, but the observed variability in a natural population suggests that a diagnostic RAPD profile for each species throughout its geographic range is unlikely.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 030021355

Download citation: RISBibTeXText


Related references

An evaluation of random amplified polymorphic DNA (RAPD) for the identification and phylogeny of freshwater snails of the genus Bulinus (Gastropoda: Planorbidae). Journal of Molluscan Studies 62(2): 165-176, 1996

Observations on shell morphology, enzymes and random amplified polymorphic DNA in Bulinus africanus group snails in Zanzibar. Journal of Molluscan Studies 63(4): 489-503, 1997

Observations on shell morphology, enzymes and random amplified polymorphic DNA (RAPD) in Bulinus africanus group snails (Gastropoda: Planorbidae) in Zanzibar. Journal of Molluscan Studies 63(4): 489-503, 1997

Detection of genetic diversity within snail intermediate hosts of the genus Bulinus by using random amplified polymorphic DNA markers (RAPDs). Acta Tropica 55(4): 205-215, 1993

Molecular phylogeny of Japanese and Taiwanese stag beetles of the genus Dorcus and allied genera (Coleoptera, Lucanidae) based on random amplified polymorphic DNA. Giornale Italiano di Entomologia 10(50): 133-140, 2002

Genotypic identification and characterization of species and strains within the genus Candida by using random amplified polymorphic DNA. Journal of Clinical Microbiology 30(12): 3249-3254, 1992

Phylogenetic analysis and the bulbous-specific band identification using random amplified polymorphic DNA in the genus Iris. Journal of the Korean Society for Horticultural Science 44(2): 228-232, 2003

An identification in fish of the genus Puntius Hamilton 1822 of some wetlands in Northeast Thailand with the use of random amplified polymorphic DNA technique. Pakistan Journal Of Biological Sciences: 4, 525-531, 2008

Evaluation of random amplified polymorphic DNA for species identification and phylogenetic analysis inStylosanthes(Fabaceae). Plant Systematics and Evolution 211(3-4): 201-216, 1998

Evaluation of random amplified polymorphic DNA for species identification and phylogenetic analysis in Stylosanthes (Fabaceae). Plant Systematics and Evolution 211(3/4): 201-216, 1998

An identification in fish of the genus Puntius Hamilton 1822 (Cypriniformes: Cyprinidae) of some wetlands in northeast Thailand with the use of random amplified polymorphic DNA technique. Pakistan Journal of Biological Sciences 11(4): 525-531, 2008

Molecular characterization of freshwater snails in the genus Bulinus: a role for barcodes?. Parasites and Vectors 1(1): 15, 2008

Blueberry Cultivar Identification Using Random Amplified Polymorphic DNA and Sequence-characterized Amplified Region Markers. Hortscience 52(11): 1483-1489, 2017

Hybrid Origin of Polyploidy in Freshwater Snails of the Genus Bulinus (Mollusca: Planorbidae). Evolution; International Journal of Organic Evolution 37(3): 592-600, 1983

Hybrid origin of polyploidy in freshwater snails of the genus Bulinus (Mollusca: Planorbidae). Evolution 37: 2-600, 1983