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Analysis of chromosome mosaicism in preimplantation embryos by using 2 sequential rounds of fluorescence in situ hybridization


Analysis of chromosome mosaicism in preimplantation embryos by using 2 sequential rounds of fluorescence in situ hybridization



Zhonghua Yi Xue Yi Chuan Xue Za Zhi 24(6): 706-708



ISSN/ISBN: 1003-9406

PMID: 18067090

Objective To investigate the mechanism and factors affecting mosaicism in human preimplantation embryos by using 2 sequential rounds of fluorescence in situ hybridization(FISH). Methods Totally 51 normal fertilized embryos, which were not suitable for embryo transfer and cryopreservation, were analyzed on day 3 after fertilization by using two sequential rounds of FISH. Chromosomes 13, 16, 18, 21, 22, X and Y were analyzed. Results Among 51 embryos, 16(31.4%) were mosaic, 12 (23.5%) were chaotic, and the remaining were either normal (27.5%)or non-mosaic abnormal(17.6%). The incidence of mosaic embryos was related to embryo developmental stage, for the incidence of mosaicism increased from 12.5% in embryos <= 4 cell stage to 40.0% in 5-8 cell stage embryos. The aneuploidy rate for the patients over 35 years of age was significantly higher than that of the patients under 35 years (57.1% vs 23.3%). Conclusion Mosaicism is common in human preimplantaion embryos, which may be one of the important factors affecting the success rates in IVF-ET. Most of the chromosomal abnormalities can be identified by two sequential rounds of FISH.

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Accession: 030054078

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