Appearance of novel homeotic CMS types in cybrids Nicotiana and Nicotiana constructed by protoplast fusion

Zubko, M.K.; Zubko, E.I.; Gleba, Y.Y.; Schieder, O.

Genetika 31(10): 1404-1412


ISSN/ISBN: 0016-6758
Accession: 030146285

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Cytoplasmic hybrids (cybrids) of tobacco with cytoplasms of Hyoscyamus niger L. and Scopolia carniolica Jacq. were constructed by protoplast fusion. In cybrids of both combinations, cytoplasmic male sterility (CMS) was revealed. Most cybrids expressed a new "green flower" trait characterized by a complete deficiency of corolla and anthers. Backcross data together with cytogenetic and restriction, fragment length polymorphism (RFLP) analyses of the cytoplasmic organelle DNA showed that cytoplasm was involved in the genetic control of this trait (maternal inheritance). The appearance of new types of CMS homeosis in alloplasmatic tobacco cybrids is discussed in relation to the possible role of nuclear-cytoplasmic interactions in the functioning of plant homeotic genes.