CDNA cloning and construction of RNAi plant expression vector of cryptochrome gene CRY2
ZhouBo; SunMei; YanShuHua; LiYuHua
Molecular Plant Breeding: 6, 1182-1186
Homological primers were designed according to the conservative cDNA sequences of CRY2 in Arabidopsis thaliana (accession number: NM_100320), Chinese cabbage (AY176689) and Brassica napus (AJ889779) in Genbank. CRY2 gene was amplified from B. napus subsp. rapa Tsuda using the designed primers by RT-PCR (reverse transcription-polymerase chain reaction). A 400 bp gene fragment was obtained and designated as BrCRY2.