EurekaMag.com logo
+ Site Statistics
References:
47,893,527
Abstracts:
28,296,643
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Carbon dioxide modified subcritical water chromatography


, : Carbon dioxide modified subcritical water chromatography. Journal of Chromatography. A 1200(1): 49-54

A novel method of increasing the elution strength in subcritical water chromatography (SWC) by adding CO2 to the water mobile phase is presented. Since the polarity of water reduces dramatically with increasing temperature, this property is used in SWC to create an isocratic mobile phase with tunable elutropic strength in reversed-phase separations. Unfortunately, thermal stability of the stationary phase dictates the upper temperature limit and therefore also the minimum available mobile phase polarity. As a result SWC is often not very effective at eluting non-polar analytes. However, when CO2 is blended into subcritical water, a considerable reduction in mobile phase polarity results and improves such separations. For example, in conventional SWC1-octanol is not observed to elute from a PRP-1 column after several hours at the maximum column temperature of 200 degrees C. In contrast to this, when CO2 is present at 180 atm (1 atm = 101 325 Pa) in the mobile phase, 1-octanol elutes with good peak shape in less than 4 min at only 100 degrees C. The technique is applied to the separation of a variety of analytes which have previously been challenging or even not possible to analyze by conventional SWC. Further, the ability to use temperature and composition programming with the blended CO2/water mobile phase in SWC is also presented and discussed. Overall, the developed method considerably extends the range of non-polar analytes amenable to SWC analysis, while maintaining the beneficial conventional SWC features of flame ionization detection and environmental compatibility.


Accession: 030409145

PMID: 18384798

DOI: 10.1016/j.chroma.2008.03.040

Submit PDF Full Text: Here


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Related references

West, C.; Lesellier, E.; Tchapla, A., 2004: Retention characteristics of porous graphitic carbon in subcritical fluid chromatography with carbon dioxide-methanol mobile phases. Numerous relationships usually used in high-performance liquid chromatography (HPLC) for describing the retention on porous graphitic carbon (PGC) have been applied in subcritical fluid chromatography, with CO2-methanol mobile phases. As reported...

Funada, Y.; Hirata, Y., 1997: Retention behavior of triglycerides in subcritical fluid chromatography with carbon dioxide mobile phase. The retention behavior of triglycerides (TGs) was studied in subcritical fluid chromatography (SubFC) at 150 atm and in the temperature range of 0 to 25 degree C using an octadecyl-silica (ODS) column and carbon dioxide as the mobile phase without...

Zhang, H.; Singer, S.J., 2011: Analysis of the subcritical carbon dioxide-water interface. We follow the evolution of the H(2)O/CO(2) interface at 300 K from the low pressure limit to near-critical pressures in molecular dynamics simulations using the SPC water and EPM2 carbon dioxide models. The intrinsic structure of the interface is...

Ibanez, E.; Lopez Sebastian, S.; Tabera, J.; Reglero, G., 1998: Separation of carotenoids by subcritical fluid chromatography with coated, packed capillary columns and neat carbon dioxide. Different packed capillary columns were evaluated for carotenoid separation by subcritical fluid chromatography with neat CO2. Packed capillary columns studied involved the use of silica particles deactivated with different methods and particles c...

Pyo, Dongjin, 2000: Separation of vitamins by supercritical fluid chromatography with water-modified carbon dioxide as the mobile phase. Supercritical fluid chromatography (SFC) has become a technique for solving problems that are difficult to be monitored by other chromatographic methods. However, the most widely used fluid, is no more polar than hexane. Polar samples which are di...

Moreschi, Sânia.R.M.; Petenate, A.J.; Meireles, M.Angela.A., 2004: Hydrolysis of ginger bagasse starch in subcritical water and carbon dioxide. Ginger bagasse from supercritical extraction was hydrolyzed using subcritical water and CO(2) to produce reducing sugars and other low molecular mass substances. Response surface methodology was used to find the best hydrolysis conditions; the deg...

Lamm, L.J.; Yang, Y., 2003: Off-line coupling of subcritical water extraction with subcritical water chromatography via a sorbent trap and thermal desorption. In this study, the off-line coupling of subcritical water extraction (SBWE) with subcritical water chromatography (SBWC) was achieved using a sorbent trap and thermal desorption. The sorbent trap was employed to collect the extracted analytes duri...

Gurdale, K.; Lesellier, E.; Tchapla, A., 2000: Study of dead volume measurement in packed subcritical fluid chromatography with ODS columns and carbon dioxide-modifier mobile phases. Studies were done for providing a simple, rapid and reliable procedure of void volume measurement in packed subcritical fluid chromatography (pSubFC), with CO2-modifier mobile phases containing high modifier amounts. Methods used in RPLC with ODS...

Pross, S.; Gau, W.; Wenclawiak, B.W., 2001: Extraction of polychlorinated biphenyl with supercritical carbon dioxide, sulfur hexafluoride and subcritical water. In the extraction of spiked PCB from soil, three extracting fluids were investigated: supercritical carbon dioxide (CO2), supercritical sulfur hexafluoride (SF6) and subcritical water. Among the tested fluids SF6 appeared to be appropriate especia...

Pross, S.; Gau, W.; Wenclawiak, B.W., 2000: Extraction of polychlorinated biphenyl with supercritical carbon dioxide, sulfur hexafluoride and subcritical water. In the extraction of spiked PCB from soil, three extracting fluids were investigated: supercritical carbon dioxide (CO2), supercritical sulfur hexafluoride (SF6) and subcritical water. Among the tested fluids SF6 appeared to be appropriate especia...