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Cell-, tissue-, and position-specific monoclonal antibodies against the planarian Dugesia tigrina






Histochemistry & Cell Biology 107(2): 139-149

Cell-, tissue-, and position-specific monoclonal antibodies against the planarian Dugesia tigrina

To obtain specific immunological probes for studying molecular mechanisms involved in cell renewal, cell differentiation, and pattern formation in intact and regenerating planarians, we have produced a hybridoma library specific for the asexual race of the freshwater planarian Dugesia (Girardia) tigrina. Among the 276 monoclonal antibodies showing tissue-, cell-, cell subtype-, subcellular- and position-specific staining, we have found monoclonal antibodies against all tissues and cell types with the exception of neoblasts, the undifferentiated totipotent stem-cells in planarians. We have also detected position-specific antigens that label anterior, central, and posterior regions. Patterns of expression uncovered an unexpected heterogeneity among previously thought single cell types, as well as interesting cross-re-activities that deserve further study. Characterization of some of these monoclonal antibodies suggests they may be extremely useful as molecular markers for studying cell renewal and cell differentiation in the intact and regenerating organism, tracing the origin, lineage, and differentiation of blastema cells, and characterizing the stages and mechanisms of early pattern formation. Moreover, two position-specific monoclonals, the first ones isolated in planarians, will be instrumental in describing- in molecular terms how the new pattern unfolds during regeneration and in devising the pattern formation model that best fits classical data on regeneration in planarians.


Accession: 030440319



Related references

Bueno, D.; Baguñà, J.; Romero, R., 1997: Cell-, tissue-, and position-specific monoclonal antibodies against the planarian Dugesia (Girardia) tigrina. To obtain specific immunological probes for studying molecular mechanisms involved in cell renewal, cell differentiation, and pattern formation in intact and regenerating planarians, we have produced a hybridoma library specific for the asexual ra...

Romero, R.; Fibla, J.; Bueno, D.; Sumoy, L.; Aureli Soriano, M.; Baguna, J., 1991: Monoclonal antibodies as markers of specific cell types and regional antigens in the freshwater planarian Dugesia (G.) tigrina. We have produced monoclonal antibodies (mAb's) against antigens of the fresh-water planarian Dugesia (G.) tigrina (Girard) using standard protocols. Labeling these mAb's with PAP (peroxidase-antiperoxidase) and indirect-immunofluorescenc...

Bueno, D.; Baguñà, J.; Romero, R., 1996: A central body region defined by a position-specific molecule in the planarian Dugesia (Girardia) tigrina: spatial and temporal variations during regeneration. We have isolated a monoclonal antibody, TCEN49, that displays position-specific binding to the central body region of the planarian Dugesia (Girardia) tigrina. TCEN49 binds to all cell types of the central area with the exception of epithelial cel...

Bueno, D.; Baguna, J.; Romero, R., 1996: A central body region of defined by a position-specific molecule in the planarian Dugesia (Girardia) tigrina: Spatial and temporal variations during regeneration. We have isolated a monoclonal antibody, TCEN49, that displays position-specific binding to the central body region of the planarian Dugesia (Girardia) tigrina. TCEN49 binds to all cell types of the central area with the exception of epithelial cel...

Bueno, D.; Batlle, E.; Soriano, M.A.reli; Espinosa, L.; Baguna, J.; Romero, R., 1995: TCAV-1, a monoclonal antibody specific to epithelial pharyngeal cells in the planarian Dugesia tigrina Application to pattern formation of the pharynx during regeneration. Hydrobiologia 305(1-3): 263-264

Collet J.; Auladell M.C.; Baguna J., 1984: Specific protein marker of neoblasts and differentiated cells in the planarian dugesia tigrina. Journal of Embryology & Experimental Morphology 82(SUPPL): 246

Collet J.; Auladell M.C.; Baguna J., 1985: Specific protein markers of neoblasts and differentiated cells in the planarian dugesia tigrina. Archives d'Anatomie Microscopique et de Morphologie Experimentale 73(4): 308

Hauschka, T., 2011: Absence of axial gradation in the tissue-differential susceptibility of the planarian, Dugesia tigrina, to hexenolactone. Anatomical Record 94: 424-424

Vowinckel C., 1970: Stimulation of germ cell proliferation in the planarian dugesia tigrina. Journal of Embryology & Experimental Morphology 23(2): 407-418

Shirakawa, T.; Sakurai, A.; Inoue, T.; Sasaki, K.; Nishimura, Y.; Ishida, S.; Teshirogi, W., 1991: Production of cell- and tissue-specific monoclonal antibodies in the freshwater planarian Phagocata vivida. Hydrobiologia, 227: 81-91