Cloning of E2 gene of classical swine fever virus and construction of recombinant adenovirus expression vector

HanXiangMin; ChengXiaoLei

Chinese Veterinary Science: 9, 782-786


Accession: 030580350

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The full length E2 gene of classical swine fever virus (CSFV) was amplified and cloned by RT-PCR. Results showed that the E2 gene contained 1100 bp, encoding 367 amino acids. Then, it was subcloned into the adenovirus shuttle vector pAdTrack-CMV and the transfer vector pAdTrack-CMV-E2 was constructed. After being linearized, the pAdtrack-CMV-E2 was transformed into E. coli strain BJ5183 competent cells.