Comparison of three DNA extraction methods for Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium
Amaro, A.; Duarte, E.; Amado, A.; Ferronha, H.; Botelho, A.
Letters in Applied Microbiology 47(1): 8-11
2008
ISSN/ISBN: 0266-8254
PMID: 18498320
DOI: 10.1111/j.1472-765x.2008.02372.x
Accession: 030649707
Aims: To compare three methods for DNA extraction from Mycobacterium bovis, Mycobacterium tuberculosis and Mycobacterium avium subsp. avium. Methods and Results: The DNA was extracted from mycobacterial cultures using enzymatic extraction, combined bead beating and enzymatic extraction and cetyltrimethylammonium bromide (CTAB) extraction. The yield and quality of DNA were compared by spectrophotometry, agarose gel electrophoresis, restriction endonuclease analysis and PCR.