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Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions



Detection of group 1 coronaviruses in bats using universal coronavirus reverse transcription polymerase chain reactions



Methods in Molecular Biology 454: 13-26



The zoonotic transmission of SARS coronavirus from animals to humans revealed the potential impact of coronaviruses on mankind. This incident also triggered several surveillance programs to hunt for novel coronaviruses in human and wildlife populations. Using classical RT-PCR assays that target a highly conserved sequence among coronaviruses, we identified the first coronaviruses in bats. These assays and the cloning and sequencing of the PCR products are described in this chapter.

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Accession: 030852405

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PMID: 19057871


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