Effects of Shaoqiduogan on cellular immunity in mice with delayed-type hypersensitivity
Fang-Sun; Wang-Meng; Zhao-Xiao-Juan; Chen-Gen-Lin; Wei-Wei
Zhongguo Yaolixue Tongbao 24(4): 444-448
2008
Accession: 031133689
Aim To investigate the effects of Shaoqiduogan (SQDG) on cellular immunity in mice with delayed-type hypersensitivity (DTH). Methods DTH model induced by 2, 4-dinitrochlorobenzene (DNCB)and cyclophosphamide (Cy) suppressed and potentiated DTH model were used. SQDG(30, 60 and 120 mg.kg(-1) were given intragastrically (ig) on cc daily for 7 consecutive days. Concanavalin A(Con A)-induced Tcells proliferation was measured by MTT assay. The activity of IL-2 in culture supernatants of Con A-induced T cells proliferation was measured by testing its ability to support the proliferation of Con A stimulated thymocytes which was detected by MTT assay. Results In mice with DTH, SQDG (120 mg . kg(-1))reduced ear swelling, thymus index, Con A-induced T cells proliferation and the activity of IL-2 in culture supernatants of Con A-induced T cells proliferation. The suppression and potentiation of DTH in mice were induced by given an intraperitoneal (ip) injection of Cy at the dose of 150 mg . kg(-1) on primary sensitization day and 250 mg -kg- I three days before primary sensitization. SQDG (60 and 120 mg . kg(-1)) upregulated ear swelling, Con A-induced T cells proliferation and the activity of IL-2 in culture supernatants of T cells from mice with Cy-suppressed DTH; and downregulated levels of the aforementioned indexes in mice with Cy-potentiated DTH. Conclusion SQDG possesses a dual modulatory effect on cellular immunity in mice.