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Embryogenesis and plantlet formation in tissue culture of Kaempferia galanga L A medicinal plant



Embryogenesis and plantlet formation in tissue culture of Kaempferia galanga L A medicinal plant



Phytomorphology 42(3-4): 253-256, 1992 ( )



Rhizome explants of Kaempferia galanga with vegetative buds developed highly embryogenic callus when cultured in MS medium supplemented with BAP + 2,4-D (0.5mg + 1.0mg/l). Callus subcultured on MS medium supplemented with BAP + NAA (0.1mg/l + 1.0mg/l) produced small globular embryoids, on the peripheral layers, which further developed into plants when transferred to hormone-free medium. Plantlets were acclimatised and subsequently transferred to field.

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Accession: 031224120

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