+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Endonucleolytic cleavage of RNA at 5' endogenous stem structures by human flap endonuclease 1

Endonucleolytic cleavage of RNA at 5' endogenous stem structures by human flap endonuclease 1

Biochemical and Biophysical Research Communications 251(2): 501-508

Structure-specific nucleases called 5' flap endonucleases cleave unannealed 5' arms of template-primer DNA model substrates at the start of the duplex and are involved in Okazaki fragment processing during DNA synthesis. To determine the possible use of the enzymes in RNA structure analysis, the cleavage of synthetic and native RNAs was examined using flap endonuclease 1 (Fen1) of HeLa cells. RNAs are cleaved at about 20% of the rate of DNA model substrates, and most of the cleavage sites are within 200 nucleotides of the 5' end. Hydrolysis of MFA2 mRNA of yeast shows that the cleavages are at the start of five possible stem structures of a folded secondary structure predicted on the basis of both chemical and enzymatic structure probing. 16S ribosomal RNA of Escherichia coli is cleaved at several 5' stem structures of its phylogenetically predicted folded structure. This type of RNA cleavage specificity may be very useful in secondary structure analysis in the future and also may be used by cells for specific 5' end-geared RNA cleavages.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 031236716

Download citation: RISBibTeXText

PMID: 9792803

DOI: 10.1006/bbrc.1998.9499

Related references

Cleavage of insertion/deletion mismatches, flap and pseudo-Y DNA structures by deoxyinosine 3'-endonuclease from Escherichia coli. Journal of Biological Chemistry 271(48): 30672-6, 1996

Substrate specificity of the ultraviolet-endonuclease from Micrococcus luteus. Endonucleolytic cleavage of depurinated DNA. European Journal of Biochemistry 69(1): 265-272, 1976

The structural basis of damaged DNA recognition and endonucleolytic cleavage for very short patch repair endonuclease. Nucleic Acids Research 29(18): 3775-3783, 2001

Endonucleolytic cleavage of polyoma virus DNA: general properties and site specificity of the virion-associated endonuclease. Cold Spring Harbor Symposia on Quantitative Biology 39 Pt 1: 247-254, 1975

Mechanism of tracking and cleavage of adduct-damaged DNA substrates by the mammalian 5'- to 3'-exonuclease/endonuclease RAD2 homologue 1 or flap endonuclease 1. Journal of Biological Chemistry 271(47): 29624-29631, 1996

Werner syndrome protein interacts with human flap endonuclease 1 and stimulates its cleavage activity. Embo Journal 20(20): 5791-5801, 2001

The flexible loop of human FEN1 endonuclease is required for flap cleavage during DNA replication and repair. EMBO (European Molecular Biology Organization) Journal 21(21): 5930-5942, 2002

Cleavage of cruciform structures by human placental endonuclease. Journal of Cellular Biochemistry Suppl. (13 Part D): 106, 1989

Arginine residues 47 and 70 of human flap endonuclease-1 are involved in DNA substrate interactions and cleavage site determination. Journal of Biological Chemistry 277(27): 24659-24666, 2002

Cleavage specificity of Saccharomyces cerevisiae flap endonuclease 1 suggests a double-flap structure as the cellular substrate. Journal of Biological Chemistry 277(17): 14379-14389, 2002

Trinucleotide repeat deletion via a unique hairpin bypass by DNA polymerase β and alternate flap cleavage by flap endonuclease 1. Nucleic Acids Research 41(3): 1684-1697, 2013

Development of quantitative DNA cleavage assay for XPG endonuclease activity using endogenous nuclear proteins in human cell lines. Oncology Reports 26(5): 1235-1241, 2011

A 55-kDa endonuclease of mammalian mitochondria: comparison of its subcellular localization and endonucleolytic properties with those of endonuclease G. Acta Medica Okayama 51(2): 55-62, 1997

Human flap endonuclease structures, DNA double-base flipping, and a unified understanding of the FEN1 superfamily. Cell 145(2): 198-211, 2011