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Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes



Engineering infectious cDNAs of coronavirus as bacterial artificial chromosomes



Methods in Molecular Biology 454: 275-291



The construction of coronavirus (CoV) infectious clones had been hampered by the large size of the viral genome (around 30 kb) and the instability of plasmids carrying CoV replicase sequences in <i>Escherichia coli</i>. Several approaches have been developed to overcome these problems. Here we describe the engineering of CoV full-length cDNA clones using bacterial artificial chromosomes (BACs).

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Accession: 031247716

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PMID: 19057870


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