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Expression, purification and characterization of anti-BAFF antibody secreted from the yeast Pichia pastoris


Expression, purification and characterization of anti-BAFF antibody secreted from the yeast Pichia pastoris



Biotechnology Letters 30(6): 1075-1080



ISSN/ISBN: 0141-5492

PMID: 18259874

DOI: 10.1007/s10529-008-9657-x

B-Cell activating factor (BAFF) is critical for B cell survival and maturation; excessive expression of it corrupts B-cell tolerance and may lead to autoimmunity. The gene, scFv-Fc, coding for the antibody of BAFF was inserted into the eukaryotic expression vector, pPICZalphaA, and transformed into Pichia pastoris. A high-level expression strain was obtained using a 'yeastern blotting' method. The scFv-Fc antibody was purified and 56 mg was obtained from 1 l of culture supernatant. It retained high binding activity to both soluble BAFF and membrane-bound BAFF.

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Accession: 031393558

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