Fluorescence in situ hybridization analysis of the interactions between honeybee larvae and Paenibacillus larvae, the causative agent of American foulbrood of honeybees

Yue,D.; Nordhoff,M.; Wieler,L.H.; Genersch,E.

Environmental Microbiology: 6, 1612-1620

2008


ISSN/ISBN: 1462-2912
DOI: 10.1111/j.1462-2920.2008.01579.x
Accession: 031472980

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Abstract
American foulbrood (AFB) is a bacterial disease of honeybee larvae caused by the spore-forming bacterium Paenibacillus larvae. Although AFB and its aetiological agent are described now for more than a century, the general and molecular pathogenesis of this notifiable disease is poorly understood. We used fluorescence in situ hybridization (FISH) performed with P. larvae-specific, 16S rRNA-targeted oligonucleotide probes to analyse the early steps in the pathogenesis of American foulbrood.