Fluorescence in situ hybridization on metaphase chromosomes with biotinylated probes. in situ hybridization, biotin labeling, cosmids, gene mapping, oncogene amplification

Cherif, D.; Derré, J.; Berger, R.

Nouvelle Revue Francaise d'Hematologie 32(6): 459-460

1990


ISSN/ISBN: 0029-4810
PMID: 2101880
Accession: 031473054

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Abstract
In situ hybridization on metaphase chromosomes have been used to localize specific nucleic acid sequences. Initially, the nucleic acid probes were labeled isotopically. Over the past few years, non isotopic techniques have considerably progressed, and are being applied to a large spectrum of biological and clinical problems.