Gene cloning, sequence analysis and eukaryotic expression vector construction of gB gene of chicken infectious laryngotracheitis virus Henna isolate

LiaoZhongLei; ChenHongYing; LiXinSheng; CuiBaoAn; LiXiangRui

Journal Of Northwest A and F University: 10, 29-33

2008


Accession: 031546782

Download citation:  
Text
  |  
BibTeX
  |  
RIS

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

Abstract
This research was done to construct a eukaryotic expression vector of infectious laryngotracheitis virus (ILTV). One pair of primers was designed and synthesized according to the chicken ILTV gB gene nucleotide sequence (M64927) published in GenBank. The gB gene of ILTV Henan isolate (ILTV-XY) was amplified by PCR from the viral DNA extracted from the chicken chorioallantoic membrane infected by ILTV-XY.