+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Infusion of UVB-treated splenic stromal cells induces suppression of beta cell antigen-specific T cell responses in NOD mice



Infusion of UVB-treated splenic stromal cells induces suppression of beta cell antigen-specific T cell responses in NOD mice



Journal of Autoimmunity 30(4): 283-292



Our previous study has demonstrated that transfusion of UVB-irradiation-induced apoptotic beta cells effectively prevents type 1 diabetes (T1D) in non-obese diabetic (NOD) mice. However, the limitation of beta cell source would preclude the clinical application of this approach. Therefore, in the present study, we have attempted to establish a more practical approach by utilizing apoptotic non-beta cells to prevent T1D. We find that apoptotic splenic stromal cells significantly suppress beta cell antigen-reactive T cell proliferation in vitro and in vivo. Moreover, beta cell antigen-specific T cells primed by beta cell antigens in the presence of apoptotic stromal cells have markedly reduced responsiveness to the re-stimulation of the same beta cell antigen. We also find that beta cell antigen-specific IL-10-producing CD4+ T cells are induced in the presence of apoptotic splenic stromal cells. As expected, transfusion of apoptotic stromal cells effectively protected NOD mice from developing T1D. Furthermore, the proliferation of adoptively transferred beta cell antigen-specific TCR-transgenic T cells in pancreatic draining lymph nodes is markedly suppressed in UVB-stroma-treated mice, indicating that UVB-stroma treatment induces immune tolerance to multiple beta cell antigens. This study provides an effective and convenient approach for managing T1D by utilizing apoptotic non-beta cells.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 031938953

Download citation: RISBibTeXText

PMID: 18226498

DOI: 10.1016/j.jaut.2007.11.017


Related references

Control of IgE responses: V. Oral administration of a synthetic derivative of the inner bacterial cell wall (SDZ 280.636) to sensitized mice induces isotype specific suppression of peak hapten specific IgE antibody forming cell responses, serum IgE levels and immediate hypersensitivity responses. Immunological Investigations 27(1-2): 105-120, 1998

Suppression of splenic plaque forming cell responses in mice treated with l 1210 tumor cell products. Abstracts of the Annual Meeting of the American Society for Microbiology 74: 105, 1974

A subset of splenic macrophages process and present native antigen to naive antigen-specific CD4+ T-cells from mice transgenic for an alpha beta T-cell receptor. Cellular Immunology 166(1): 62-70, 1995

Antigen, in the presence of TGF-beta, induces up-regulation of FoxP3gfp+ in CD4+ TCR transgenic T cells that mediate linked suppression of CD8+ T cell responses. Journal of Immunology 179(4): 2105-2114, 2007

Determinant specific suppression of antigen-induced T cell proliferation in the guinea pig. I. Quantitation of suppressed antigen-specific T cell responses as a consequence of prior exposure to antigen in incomplete Freund's adjuvant. Journal of Immunology 124(1): 184-188, 1980

Haplotype-specific suppression of antibody responses in vitro. Ii. Suppressor factor produced by T cells and T cell hybridomas from mice treated as neonates with semiallogeneic spleen cells. Journal of Experimental Medicine 154(1): 48-59, 1981

A syngeneic splenic cell antigen induces a suppressor T cell in lymph nodes that controls cytotoxic T-cell and primary antibody responses. Cellular Immunology 105(1): 205-219, 1987

Haplotype specific suppression of antibody responses in vitro 2. suppressor factor produced by t cells and t cell hybridomas from mice treated as neo nates with semi allogeneic spleen cells. Journal of Experimental Medicine 154(1): 48-59, 1981

Splenic Dendritic Cells from Antigen-Fed Mice Induced Antigen-Specific T Cell Unresponsiveness in vivo. Cytotechnology 43(1-3): 41-48, 2003

Splenic Dendritic Cells from Antigen-Fed Mice Induced Antigen-Specific T Cell Unresponsiveness in vivo. Cytotechnology 43(1-3): 41-48, 2003

Antigen-specific interactions between human CD4+ T cell clones and IFNgamma-treated MHC class II+ human mesangial cells modify T cell responses to professional antigen presenting cells. Journal of the American Society of Nephrology 10(PROGRAM AND ABSTR ISSUE): 515A, 1999

Antigen-specific suppression of human antibody responses by allogeneic T cells. II. Cell interactions involved in the generation of suppression. European Journal of Immunology 16(3): 252-256, 1986

Interferon-beta induces selective enhancement of antigen-specific T cell responses. Journal of Interferon and Cytokine Research 20(4): 383-389, 2000

Determinant specific suppression of antigen-induced T cell proliferation in the guinea pig. II. Determinant specific suppression of in vitro T cell responsiveness parallels a selective suppression of anti-hapten but not anti-carrier antibody responses. Journal of Immunology 124(1): 189-193, 1980

Antigen-specific T-cell-mediated suppression. I. Induction of L-glutamic acid60-L-alanine30-L-tyrosine10 specific suppressor T cells in vitro requires both antigen-specific T-cell-suppressor factor and antigen. Journal of Experimental Medicine 147(1): 123-136, 1978