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Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses

Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses

Methods in Molecular Biology 454: 229-236

Targeted RNA recombination has proven to be a powerful tool for the genetic engineering of the coronavirus genome, particularly in its 3' part. Here we describe procedures for the generation of recombinant and mutant mouse hepatitis virus and feline infectious peritonitis virus. Key to the two-step method is the efficient selection of recombinant viruses based on host cell switching.

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Accession: 032246105

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PMID: 19057874

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