EurekaMag.com logo
+ Site Statistics
References:
48,252,460
Abstracts:
22,798,453
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Mitochondrial dysfunction in lyssavirus-induced apoptosis


, : Mitochondrial dysfunction in lyssavirus-induced apoptosis. Journal of Virology 82(10): 4774-4784

Lyssaviruses are highly neurotropic viruses associated with neuronal apoptosis. Previous observations have indicated that the matrix proteins (M) of some lyssaviruses induce strong neuronal apoptosis. However, the molecular mechanism(s) involved in this phenomenon is still unknown. We show that for Mokola virus (MOK), a lyssavirus of low pathogenicity, the M (M-MOK) targets mitochondria, disrupts the mitochondrial morphology, and induces apoptosis. Our analysis of truncated M-MOK mutants suggests that the information required for efficient mitochondrial targeting and dysfunction, as well as caspase-9 activation and apoptosis, is held between residues 46 and 110 of M-MOK. We used a yeast two-hybrid approach, a coimmunoprecipitation assay, and confocal microscopy to demonstrate that M-MOK physically associates with the subunit I of the cytochrome c (cyt-c) oxidase (CcO) of the mitochondrial respiratory chain; this is in contrast to the M of the highly pathogenic Thailand lyssavirus (M-THA). M-MOK expression induces a significant decrease in CcO activity, which is not the case with M-THA. M-MOK mutations (K77R and N81E) resulting in a similar sequence to M-THA at positions 77 and 81 annul cyt-c release and apoptosis and restore CcO activity. As expected, the reverse mutations, R77K and E81N, introduced in M-THA induce a phenotype similar to that due to M-MOK. These features indicate a novel mechanism for energy depletion during lyssavirus-induced apoptosis.

(PDF same-day service: $19.90)

Order PDF Full Text

Close
Close

Click here to order any other PDF Full Text


Accession: 032358927

PMID: 18321977

DOI: 10.1128/JVI.02651-07

PDF Full Text: Submit


Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:
Close
Close

Other references

Ates, RML., 1981: More about the butter fish.. Het Zee-Aquarium, 312: 19-20

Hyman, MAM.; Mowbray, WH.; Saila, SB., 1975: The effects of two electrical barriers on the entrainment of fish at a freshwater nuclear power plant. Unknown

Saito, M., 1990: Transplanting cultivation of emerging rice seedlings. 1. Transplanting date and percentage of seedling establishment and heading date. In trials in 1989-90, rice seedlings were transplanted 2, 3 or 4 d after emergence on various dates between 18 Apr. and 18 May and were transplanted 1 or 3 cm deep. The percentage of missing plants was higher and plant density lower with the deepe...

O'reilly, James; Lanigan, Bernadette; O'keefe, Michael, 2001: Long-term visual results following primary trabeculectomy for infantile glaucoma. Purpose: To report the long-term visual outcome of eyes that underwent primary trabeculectomy for infantile glaucoma and to evaluate factors that affect visual prognosis. Methods: Thirty-five eyes of 22 consecutive patients presenting between 1980...

Aronsson, H.; Combe, J.; Patel, R.; Jarvis, P., 2006: In vivo assessment of the significance of phosphorylation of the Arabidopsis chloroplast protein import receptor, atToc33. AtToc33 is a transit peptide receptor of the chloroplast outer envelope membrane, and possesses GTPase activity. In vitro, its transit peptide- and GTP-binding properties are abrogated by its phosphorylation at serine 181, which was proposed to re...

Dean, H. A., 1959: Quadrant distribution of mites on leaves of Texas grapefruit. Populations of 3 species of mites sampled from terminal-flush grapefruit leaves differed in the various quadrants from which they were collected. Eutetranychus banksi McG. was most abundant in the south quadrants during the year except for the 2 m...

Jamshid, Roozbeh; Reza, Serati-Ali; Abbas, Ghaderi; Raha, Afshariani, 2003: Incidence of arteriovenous thrombosis and the role of anticardiolipin antibodies in hemodialysis patients. Intruduction: Fistula thrombosis in patients on maintenance hemodialysis is an important morbidity factor. Arterial or venous thrombotic events have been described as complications in patients on regular hemodialysis and positive titers of anticar...

Harm, Deborah L.; Jennings, Richard T.; Meck, Janice V., 2001: Gender issues related to spaceflight: a NASA perspective. Harm, Deborah L., Richard T. Jennings, Janice V. Meck, Michael R. Powell, Lakshmi Putcha, Clarence P. Sams, Suzanne M. Schneider, Linda C. Shackelford, Scott M. Smith, and Peggy A. Whitson. Invited Review: Gender issues related to spaceflight: a N...

Yan-Fei; Zheng-Yinying; Zhang-Wenwei; Xiao-Hong; Li-Shifang; Cheng-Zhuomin, 2006: Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV. In fission yeast (Schizosaccharomyces pombe), pac1 gene was cloned with 99.3% nucleotide sequence similarity with published pact in GenBank. In pET-5 alpha expression system, the expression product of cloned pac1 in E. coli showed activity to deg...

Kiyatkin, P. F., 1976: Genetic breed differences in homeostasis of sheep. A 32 deg C, Saraja sheep had a much higher respiration rate than Hissar sheep; body temp. was 39.8 deg and 40.1 deg resp. Sweat gland density was 360 and 280/cm2 skin resp. At 3 deg -9 deg , clinical and haematological indices in the 2 breeds were...