+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Natural variation in CBF gene sequence, gene expression and freezing tolerance in the Versailles core collection of Arabidopsis thaliana



Natural variation in CBF gene sequence, gene expression and freezing tolerance in the Versailles core collection of Arabidopsis thaliana



Bmc Plant Biology 8: 105



Plants from temperate regions are able to withstand freezing temperatures due to a process known as cold acclimation, which is a prior exposure to low, but non-freezing temperatures. During acclimation, a large number of genes are induced, bringing about biochemical changes in the plant, thought to be responsible for the subsequent increase in freezing tolerance. Key regulatory proteins in this process are the CBF1, 2 and 3 transcription factors which control the expression of a set of target genes referred to as the "CBF regulon". To assess the role of the CBF genes in cold acclimation and freezing tolerance of Arabidopsis thaliana, the CBF genes and their promoters were sequenced in the Versailles core collection, a set of 48 accessions that maximizes the naturally-occurring genetic diversity, as well as in the commonly used accessions Col-0 and WS. Extensive polymorphism was found in all three genes. Freezing tolerance was measured in all accessions to assess the variability in acclimated freezing tolerance. The effect of sequence polymorphism was investigated by evaluating the kinetics of CBF gene expression, as well as that of a subset of the target COR genes, in a set of eight accessions with contrasting freezing tolerance. Our data indicate that CBF genes as well as the selected COR genes are cold induced in all accessions, irrespective of their freezing tolerance. Although we observed different levels of expression in different accessions, CBF or COR gene expression was not closely correlated with freezing tolerance. Our results indicate that the Versailles core collection contains significant natural variation with respect to freezing tolerance, polymorphism in the CBF genes and CBF and COR gene expression. Although there tends to be more CBF and COR gene expression in tolerant accessions, there are exceptions, reinforcing the idea that a complex network of genes is involved in freezing tolerance and that the CBF genes alone cannot explain all differences in phenotype. Our study also highlights the difficulty in assessing the function of single transcription factors that are members of closely related gene families.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 032488062

Download citation: RISBibTeXText

PMID: 18922165

DOI: 10.1186/1471-2229-8-105


Related references

Natural variation in Arabidopsis thaliana reveals shoot ionome, biomass, and gene expression changes as biomarkers for zinc deficiency tolerance. Journal of Experimental Botany 68(13): 3643-3656, 2018

Freezing tolerance and gene expression in cold treated arabidopsis thaliana. Plant Physiology 86(4 SUPPL): 37, 1988

Cold-regulated gene expression and freezing tolerance in an Arabidopsis thaliana mutant. Plant journal: for cell and molecular biology 17(3): 301-308, 1999

Expression of a rab/lea/dehydrin-related gene and development of freezing tolerance in Arabidopsis thaliana. Nordisk Jordbruksforskning 78(2): 82, 1996

Effect of constitutive expression of the COR15a gene on the freezing tolerance of isolated protoplasts of Arabidopsis thaliana. Cryobiology 33(6): 656-657, 1996

Ectopic expression of ABI3 gene enhances freezing tolerance in response to abscisic acid and low temperature in Arabidopsis thaliana. Plant journal: for cell and molecular biology 25(1): 1-8, 2001

Consitutive expression of the cold-regulated Arabidopsis thaliana COR15a gene affects both chloroplast and protoplast freezing tolerance. Proceedings of the National Academy of Sciences of the United States of America 93(23): 13404-13409, 1996

Clinal variation in freezing tolerance among natural accessions of Arabidopsis thaliana. New Phytologist 177(2): 419-427, 2007

Clinal Variation in Freezing Tolerance among Natural Accessions of Arabidopsis thaliana. New Phytologist 177(2): 419-427, 2008

Alterations in Water Status, Endogenous Abscisic Acid Content, and Expression of rab18 Gene during the Development of Freezing Tolerance in Arabidopsis thaliana. Plant Physiology 104(4): 1341-1349, 1994

Natural Variation in Freezing Tolerance and Cold Acclimation Response in Arabidopsis thaliana and Related Species. Advances in Experimental Medicine and Biology 1081: 81-98, 2018

Natural variation in the freezing tolerance of Arabidopsis thaliana: effects of RNAi-induced CBF depletion and QTL localisation vary among accessions. Plant Science 180(1): 12-23, 2011

Genetic and Molecular Analyses of Natural Variation Indicate Cbf2 as a Candidate Gene for Underlying a Freezing Tolerance Quantitative Trait Locus in Arabidopsis. Plant Physiology 139(3): 1304-1312, 2005

Genetic and molecular analyses of natural variation indicate CBF2 as a candidate gene for underlying a freezing tolerance quantitative trait locus in Arabidopsis. Plant Physiology 139(3): 1304-1312, 2005

Mode of action of the COR15a gene on the freezing tolerance of Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America 95(24): 14570-14575, 1998