+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Novel anti-adherence activity of mulberry leaves: inhibition of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated from Morus alba



Novel anti-adherence activity of mulberry leaves: inhibition of Streptococcus mutans biofilm by 1-deoxynojirimycin isolated from Morus alba



Journal of Antimicrobial ChemoTherapy 62(4): 751-757



The present study focused on isolation, characterization and evaluation of purified compounds from Morus alba against Streptococcus mutans biofilm formation. The effect of crude extract from M. alba leaves was evaluated against oral pathogens, chiefly S. mutans. MICs were determined by the microdilution method. The compound was purified by employing silica gel chromatography and critically analysed with GC-MS, NMR and IR spectroscopy. The S. mutans traits of adherence and biofilm formation were assessed at sub-MIC concentrations of the crude extract and purified compound. Both water-soluble and alkali-soluble polysaccharide were estimated to determine the effect of the purified compound on the extracellular polysaccharide secretion of S. mutans. Its effect on biofilm architecture was also investigated with the help of confocal microscopy. The purified compound of M. alba showed an 8-fold greater reduction of MIC against S. mutans than the crude extract (MICs, 15.6 and 125 mg/L, respectively). The extract strongly inhibited biofilm formation of S. mutans at its active accumulation and plateau phases. The purified compound led to a 22% greater reduction in alkali-soluble polysaccharide than in water-soluble polysaccharide. The purified compound was found to be 1-deoxynojirimycin (DNJ). Confocal microscopy revealed that DNJ distorts the biofilm architecture of S. mutans. CONCLUSIONS The whole study reflects a prospective role of DNJ as a therapeutic agent by controlling the overgrowth and biofilm formation of S. mutans.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 032581619

Download citation: RISBibTeXText

PMID: 18565974

DOI: 10.1093/jac/dkn253


Related references

Response surface optimized extraction of 1-deoxynojirimycin from mulberry leaves (Morus alba L.) and preparative separation with resins. Molecules 19(6): 7040-7056, 2014

Antioxidant flavonol glycosides in mulberry (Morus alba L.) leaves isolated based on LDL antioxidant activity. Food Chemistry 97(1): 25-31, 2006

The relationship between 1-deoxynojirimycin content and alpha-glucosidase inhibitory activity in leaves of 276 mulberry cultivars (Morus spp.) in Kyoto, Japan. Journal of Natural Medicines 62(1): 63-66, 2008

Phenolic-containing organic extracts of mulberry (Morus alba L.) leaves inhibit HepG2 hepatoma cells through G2/M phase arrest, induction of apoptosis, and inhibition of topoisomerase IIα activity. Journal of Medicinal Food 13(5): 1045-1056, 2010

Lipolytic effect of compounds isolated from leaves of mulberry (Morus alba L.) in 3T3-L1 adipocytes. Natural Product Research 32(16): 1963-1966, 2018

Transcriptome analysis and identification of key genes involved in 1-deoxynojirimycin biosynthesis of mulberry ( Morus alba L.). Peerj 6: E5443, 2018

Anti-diabetic effect of protoplast preparation from fresh leaves of mulberry (Morus alba). Natural Medicines 55(4): 181-186, 2001

Isolation and identification of methyl caffeate methyl 3 4 di hydroxy cinnamate from dwarf diseased mulberry morus alba cultivar ichinose leaves and its anti bacterial activity. Journal of Sericultural Science of Japan 49(1): 63-66, 1980

Immunomodulatory activity of methanolic extract of Morus alba Linn. (mulberry) leaves. Pakistan Journal of Pharmaceutical Sciences 23(1): 63-68, 2010

Apoptosis-inducing activity of ethanol extracts from the tea of mulberry (Morus alba) leaves in HL-60 cells. Nippon Shokuhin Kagaku Kogaku Kaishi = Journal of the Japanese Society for Food Science and Technology 49(3): 195-198, 2002

Anti-inflammatory and anti-cancer activity of mulberry (Morus alba L.) root bark. Bmc Complementary and Alternative Medicine 14: 200, 2014

Antibacterial activity and anti-biofilm effect of chitosan against strains of Streptococcus mutans isolated in dental plaque. International Journal of Immunopathology and Pharmacology 21(4): 993-997, 2008

Extraction optimization, characterization and antioxidant activity in vitro of polysaccharides from mulberry (Morus alba L.) leaves. Carbohydrate Polymers 128: 52-62, 2015

Evaluation of anti-inflammatory activity of prenylated substances isolated from Morus alba and Morus nigra. Journal of Natural Products 77(6): 1297-1303, 2014

(Poly)phenolic fingerprint and chemometric analysis of white (Morus alba L.) and black (Morus nigra L.) mulberry leaves by using a non-targeted UHPLC-MS approach. Food Chemistry 212: 250-255, 2016