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Preliminary evaluation of purified antigen used for the serodiagnosis of systemic candidosis by enzyme linked immunoSorbent assay

Preliminary evaluation of purified antigen used for the serodiagnosis of systemic candidosis by enzyme linked immunoSorbent assay

Journal de Mycologie Medicale 5(3): 140-144

Purpose. To improve the serological diagnosis of systemic candidiasis, we used two different purified antigens of Candida albicans to study the IgG humoral response by ELISA (Enzyme Linked ImmunoSorbent Assay). Materials and methods. The antigen 48 is a cytoplasmic antigen with an enolase activity and was purified by ion exchange chromatography. The antigen 3D9 is a mannoprotein C. albicans germtube specific antigen, purified by gel filtration and hydrophobic interaction chromatography. Sera were obtained from patients with systemic candidosis (n = 37), vaginal candidosis (n = 14), aspergillosis (n = 11) and from blood donors (n = 18). Results. Statistical analysis showed significant differences between absorbance values obtained with sera of the population of patients with systemic candidiasis and with sera of the three other populations (p lt 0.0001). On the contrary, there was no significant difference (p gt 0.3) between absorbance values obtained in populations of patients with vaginal candidosis, aspergillosis and blood donors. We obtained sensitivity and specificity values of 73 and 95,5% respectively with IgG anti-Ag48 and of 78 and 82% respectively with IgG anti-Ag3D9. Positive predictive value and negative predictive value were 90 and 80% respectively with IgG anti-Ag48 and they were 84 and 84% respectively with IgG anti-Ag3D9. Conclusion. Diagnostic parameters (specificity, sensitivity) were satisfactory and showed the usefulness of these antigens to determine IgG anti-Ag3D9 and IgG anti-Ag48 for the diagnosis of systemic candidosis.

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