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Random amplified polymorphic DNA markers as tools for gene mapping in Chlamydomonas eugametos



Random amplified polymorphic DNA markers as tools for gene mapping in Chlamydomonas eugametos



Journal of Phycology 32(6): 1043-1048



Studying the sexual behavior of the heterothallic green alga Chlamydomonas eugametos Moewus has revealed genetic differences among the few available strains: different O-methylated sugar patterns on plasma membrane glycoproteins (oms A/B locus) and light-(in)dependent sexual agglutinin activation (lsa locus). However, due to the lack of a reliable linkage map, genes controlling these traits are not accessible by map-based cloning. Here we present a partial linkage map for Chlamydomonas eugametos based on random amplified polymorphic DNA (RAPD) markers and one restriction fragment-length polymorphism (RFLP) marker. Most of these RAPDs (80%) represent unique DNA sequences, which implies that they can be used as starting points for chromosome walking. RAPDs linked to the lsa locus, the mating type locus (mt), and the oms A/B locus were identified by bulk segregant analysis.

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Accession: 033080358

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