Restriction endonuclease digestion of amplification products generated by RAPD technique in a population of Glycine soja

Wei, W.; Zhong, M.; Wang, H.X.; Yun, R.; Hu, Z.A.; Qian, Y.Q.

Acta Botanica Sinica 40(5): 412-416


ISSN/ISBN: 0577-7496
Accession: 033220486

Download citation:  

Article/Abstract emailed within 1 workday
Payments are secure & encrypted
Powered by Stripe
Powered by PayPal

In population of Glycine soja L., the polymorphic loci could be hardly detected by RAPD markers, using several primers. These non-polymorphic amplification products were cleaved by some restriction endonuclease, such as Msp I, Hinf I, Taq I, EcoRI, Sal I, Dra I and HaeM . After cleaving, the digested amplification products were detected on polyacrylamide gel electrophoresis with silver staining. It was found that: 1) mm restriction endonucleases could not, axid mm others could effectively digest the random amplication products of the DNAs of G. soja; 2) some endonucleases could produce polymorphic DNA fragments after digestion of the non-polymorphic products, but others could not even after digestion; 3) non-polymorphic amplification products amplified by some primers could produce polymorphic DNA fragments after digestion, while those by other primers could not. it could be concluded that the restriction endonuclease digestion of amplification products could increase significantly detectability of polymorphic DNA by RAPDs technique.