Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO (2) concentration

Cabálková, J.; Pribyl, J.; Skládal, P.; Kulich, P.; Chmelík, J.

Tree Physiology 28(10): 1593-1599

2008


ISSN/ISBN: 0829-318X
PMID: 18708341
DOI: 10.1093/treephys/28.10.1593
Accession: 033406907

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Abstract
We compared the effects of ambient (350 ppm) and elevated CO2 concentration (700 ppm) on the size and shape of starch granules in Norway spruce (Picea abies (L.) Karst.) needles during one growing season. Starch granules were isolated from needles by alkaline digestion and analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Measurements made with a particle size analyzer indicated that starch granules ranged between 0.5 and 10 micro m.