+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Slingshot-3 dephosphorylates ADF/cofilin but is dispensable for mouse development

Slingshot-3 dephosphorylates ADF/cofilin but is dispensable for mouse development

Genesis 46(5): 246-255

Actin-depolymerizing factor (ADF) and cofilin constitute a family of key regulators of actin filament dynamics. ADF/cofilin is inactivated by phosphorylation at Ser-3 by LIM-kinases and reactivated by dephosphorylation by Slingshot (SSH) family phosphatases. Defects in LIM kinases or ADF/cofilin have been implicated in morbidity in human or mice; however, the roles of mammalian SSH in vivo have not been addressed. In this study, we examined the endogenous expression of each mouse SSH member in various cell lines and tissues, and showed that SSH-3L protein was strongly expressed in epithelial cells. Our structure-function analysis of SSH-3L suggested the possibility that the C-tail unique to SSH-3L negatively regulates the catalytic activity of this phosphatase. Furthermore we made ssh-3 knockout mice to examine its potential in vivo roles. Unexpectedly, ssh-3 was not essential for viability, fertility, or development of epithelial tissues; and ssh-3 did not genetically modify the corneal disorder of the corn1 ADF/destrin mutant.

(PDF emailed within 0-6 h: $19.90)

Accession: 033411725

Download citation: RISBibTeXText

PMID: 18442045

DOI: 10.1002/dvg.20389

Related references

Identification and characterization of three mouse Slingshot phosphatases that dephosphorylate cofilin. Cell Structure & Function 28(4): 318, August, 2003

A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia. Journal of Cell Biology 165(4): 465-471, 2004

Formation of cofilin-actin rods following cucurbitacin-B-induced actin aggregation depends on Slingshot homolog 1-mediated cofilin hyperactivation. Journal of Cellular Biochemistry 114(10): 2415-2429, 2014

Involvement of slingshot in the Rho-mediated dephosphorylation of ADF/cofilin during Xenopus cleavage. Zoological Science 22(9): 971-984, 2005

Control of actin reorganization by Slingshot, a family of phosphatases that dephosphorylate ADF/cofilin. Cell 108(2): 3-46, 2002

Identification of multiple actin-binding sites in cofilin-phosphatase Slingshot-1L. Febs Letters 580(7): 1789-1794, 2006

Actin filaments-stabilizing and -bundling activities of cofilin-phosphatase Slingshot-1. Genes to Cells 12(5): 663-676, 2007

Calcium signal-induced cofilin dephosphorylation is mediated by Slingshot via calcineurin. Journal of Biological Chemistry 280(13): 12683-9, 2005

Reactive oxygen species regulate a slingshot-cofilin activation pathway. Molecular Biology of the Cell 20(11): 2650-2660, 2009

Cofilin and slingshot localization in the epithelium of uterine endometrium changes during the menstrual cycle and in endometriosis. Reproductive Sciences 18(10): 1014-1024, 2012

Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. Journal of Biological Chemistry 289(38): 26302-26313, 2015

Extracellular potassium deprivation reversibly dephosphorylates cofilin. Biochemical and Biophysical Research Communications 345(4): 1393-1397, 2006

A pleckstrin homology-like domain is critical for F-actin binding and cofilin-phosphatase activity of Slingshot-1. Biochemical and Biophysical Research Communications (): -, 2016

BMP gradients steer nerve growth cones by a balancing act of LIM kinase and Slingshot phosphatase on ADF/cofilin. Journal of Cell Biology 178(1): 107-119, 2007