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Somatic embryogenesis in cowpea (Vigna unguiculata (L.) Walp.)



Somatic embryogenesis in cowpea (Vigna unguiculata (L.) Walp.)



In Vitro Cellular & Developmental Biology Animal. 34(3 Part 2): 71a



A method for inducing somatic embryogenesis in cowpea [Vigna unguiculata (L.) Walp.] has been developed, starting from mature cotyledon explants. Induction of somatic embryogenesis was influenced by basal media composition, pH, hormone concentration, gelling agents and incubation environment. Cotyledons and embryos from two cultivars, Ife brown and 83D-442, were excised from green immature pods. The proximal part of the cotyledon was removed and transferred to several induction media with a range of hormone concentrations, pH and basal media for four weeks. The induced cotyledons were transferred to hormone-free liquid medium for 1 week and then transferred to the same medium containing 0.2% phytagel. Several somatic embryos developed within 6 weeks from the wounded region of the cotyledons. The embryos were isolated and dehydrated for 4 days and transferred again to the same solid medium for germination. After germination the plantlets were transferred to soil and developed into normal fertile plants. The optimised protocol was effective in both varieties.

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Accession: 033432693

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