Section 34
Chapter 33,894

The pigment production of Lactobacillus plantarum L 622 Studies on the red orange pigment producing lactic acid bacteria L 622 isolated from fermented rice noodle Khanom Jeen of Thailand Part II

Takao, T.; Niimura, Y.; Okada, S.; Ohara, N.; Uchimura, T.; Kozaki, M.

Journal of Japanese Society of Food Science and Technology 39(9): 755-762


ISSN/ISBN: 0029-0394
Accession: 033893379

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Orange pigment was found in the cell of Lactobacillus plantarum L 622 (strain L 622) isolated from fermented rice noodle "Khanom Jeen". The optimum culture condition for pigment formation was incubation at 25 degree C and pH 7.0 with Na gluconate as carbon source and cystine and methionine as amino acid source. Very little amount of pigment was found in the cell culture with glucose as carbon source. Addition of antibiotics which inhibit the protein synthesis increased the pigment formation. Pigment formation was related with the rate of growth but not with the amount of intracellular ATP. Pigment forming strain L. plantarum L 622 and pigment nonforming strain L 622 CW were not differed in the physiological characteristics. The growth of strain L 622 was stimulated by the addition of cystine or methionine while L 622 CW was not. The differece between the strain L 622 and strain L 622 CW was the pattern of plasmid DNA. Strain L 622 retained 5 bands of plasmids, 49 kbp. to 12.6 kbp. But strain L 622 CW had 11.5 klbp plasmid instead of 12.6 kbp plasmid. It suggested the pigment production of L 622 wsas related with the plasmid.

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