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Transformation of Solanum tuberosum CV BF15 by two Agrobacterium rhizogenes strains Biochemical and morphological analysis of transformed plants

Dobigny, A.; Martin, N.; Haicour, R.; Rossignol, L.

Phytomorphology (Delhi) 43(3-4): 217-225

1994


ISSN/ISBN: 0031-9449
Accession: 034045507

Discs of microtubers were used for the first time to transform a french potato (Solanum tuberosum L.) variety, BF15 using Agrobacterium rhizogenes. Plant regeneration was obtained in 6 weeks from transformed roots after production of a callus. Confirmation of the transformation events was established by opine detection and by kanamycin resistance test for both roots and shoots. Transformed plants had a distinct phenotype and development compared to untransformed control plants. Nevertheless, tuberization function was never lost. Morphological modifications of transformed plants could be due especially to the expression of the T-DNA genes integrated in plant cells. To a lesser extent, it could also be due to the intervening callus phase during regeneration process. On the contrary, only this last phenomenon seemed to be responsible for variations in ploidy level or number of chloroplasts per stomata.

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