+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Urothelium EP1 receptor facilitates the micturition reflex in mice

Urothelium EP1 receptor facilitates the micturition reflex in mice

Biomedical Research 29(2): 105-111

We investigated the presence of EP1 receptor in the urothelium and its role in micturition reflex by examining the effect of intravesical administration of prostaglandin E-2 (PGE(2)), an EP1 agonist (ONO-DI-004), acetic acid, and capsaicin. Age-matched EP1-KO mice and C57BL/6 wild-type (WT) mice were used. Western blots and standard immunohistochemical procedures were performed. Cystometrygram (CMG) was performed without anesthesia in a restraining cage. ATP release from the cultured urothelium cells was performed using luciferin-luciferase luminometry. The EP1 receptor was found to be present in the urothelium. In WT mice, PGE(2) infusion shortened the inter-contraction interval (ICI) in a dose-dependent fashion; however, it did not alter the ICI in EP1-KO mice. The EP1 agonist significantly shortened the ICI in WT mice, but not in EP1-KO mice. Acetic acid and capsaicin shortened the ICI in both WT mice and EP1-KO mice. EP1 agonist, PGE(2) and capsaicin provoked ATP release from cultured urothelial cells. These results suggest that EP1 receptor was present in bladder urothelium, and could be activated by PGE(2) to release ATP. EP1 receptor in urothelium might be important for reflex voiding in pathological conditions.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 034144722

Download citation: RISBibTeXText

PMID: 18480552

Related references

Activation of alpha1D adrenergic receptors in the rat urothelium facilitates the micturition reflex. Journal of Urology 175(1): 358-364, 2006

PACAP/VIP and receptor characterization in micturition pathways in mice with overexpression of NGF in urothelium. Journal of Molecular Neuroscience 42(3): 378-389, 2011

Effects of CYP-Induced Cystitis on Growth Factors and Associated Receptor Expression in Micturition Pathways in Mice with Chronic Overexpression of NGF in Urothelium. Journal of Molecular Neuroscience 59(4): 531-543, 2016

Effects of CYP-induced cystitis on PACAP/VIP and receptor expression in micturition pathways and bladder function in mice with overexpression of NGF in urothelium. Journal of Molecular Neuroscience 48(3): 730-743, 2013

PACAP/VIP receptor characterization and regulation with cystitis in micturition pathways and functional role for pacap in urothelium. 2007

PACAP-mediated ATP release from rat urothelium and regulation of PACAP/VIP and receptor mRNA in micturition pathways after cyclophosphamide (CYP)-induced cystitis. Journal of Molecular Neuroscience 36(1-3): 310-320, 2008

Ampa glutamate receptor associated with micturition reflex of the rat. Japanese Journal of Physiology 41(SUPPL): S279, 1991

Striated muscle activity during the micturition reflex in the rat and micturition patterns in a sexual context. Society for Neuroscience Abstracts 20(1-2): 1754, 1994

The effects of glutamate receptor antagonists on the micturition reflex in the rat. Society for Neuroscience Abstracts 17(1-2): 1001, 1991

The effects of MK-801, an NMDA receptor antagonist, on the micturition reflex in the rat. Neuroscience Letters 126(2): 141-144, 1991

Opioid modulation of the micturition reflex at the level of the pontine micturition center. Urologia Internationalis 47 Suppl 1: 19-22, 1991

Activation of the micturition reflex by NK-2 receptor stimulation in the anesthetized guinea-pig. British Journal of Pharmacology 115(6): 875-882, 1995

Anandamide increases the micturition reflex activity via the vanilloid receptor 1. Society for Neuroscience Abstract Viewer & Itinerary Planner : Abstract No 49 11, 2002

Intrathecal administration of NK1 receptor antagonist, CP96345, inhibits the micturition reflex in the rat. Regulatory Peptides 46(1-2): 392-395, 1993