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Use of chromatography for the preparation of homologous tetanus antitoxin

Use of chromatography for the preparation of homologous tetanus antitoxin

Journal of Chromatography 520: 425-431

To obtain immunoglobulin of selected action with a higher titre, techniques for isolating antibodies from a donor's normal immunoglobulin by means of affinity chromatography were used. To obtain an affinity sorbent, tetanus toxoid was used as a ligand. Using an immunosorbent based on the purified ligand, tetanus antibodies were prepared with a specific activity of 65 IU/mg. According to gel-permeation chromatography, the tetanus antibodies obtained were represented mainly by IgG monomer (greater than 90%). The anticomplementary activity of the preparations obtained, being at least 11 mg of protein per 2CH50, does not rule out the possibility of intravenous administration of tetanus antibodies.

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Accession: 034150139

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PMID: 1707888

DOI: 10.1016/0021-9673(90)85129-j

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