+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Utility of random amplified polymorphic DNA markers for linkage mapping in Turkish red pine

Utility of random amplified polymorphic DNA markers for linkage mapping in Turkish red pine

Silvae Genetica 44(2-3): 110-116

ISSN/ISBN: 0037-5349

We have applied the random amplified polymorphic DNA (RAPD) marker system to estimate linkage relationships in Pinus brutia TEN. We used DNA samples from 30 haploid seed megagametophytes from each of 4 mother trees. Ninety-five 10-base oligonucleotide primers were evaluated and 34 revealed at least one polymorphic RAPD locus. The number of segregating RAPD loci per polymorphic primer varied from 2.32 to 3.25, but when segregating loci per tested primer was considered it was low, ranging on the average, from 0.28 in tree 4 to 0.59 in tree 1. Based on the RAPD loci segregating in 1:1 ratio, genetic linkage groups formed from 6 for genotype-4 (total map distance=163.91 cM) to 13 for genotype-3 (total map distance= 511.2 cM). It was also found that a number of segregating loci in all 4 genotypes (ranging from 14 to 21) could not be assigned into any of the constructed linkage groups. It was difficult to compare linkage groups among the genotypes since most of RAPD loci segregating in one genotype were not found in others. Thus, the linkage map provided very little information on the genomic organization of RAPD markers at the species level. The utility of RAPD markers in forest genetics is also discussed in the paper.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 034168267

Download citation: RISBibTeXText

Related references

Utility of random amplified polymorphic DNA (RAPD) markers for linkage mapping in Turkish red pine (Pinus brutia Ten.). Silvae Genetica 44(2/3): 110-116, 1995

Comparison of homology and linkage of random amplified polymorphic DNA markers between individual trees of Scots pine. Molecular Ecology 8(1): 15-22, 1999

Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 89(5): 606-614, 1994

Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers. Genetics 143(4): 1727-1738, 1996

Linkage of random amplified polymorphic DNA markers in Pinus halepensis Mill. Silvae Genetica 51(5/6): 196-201, 2002

Construction of a linkage map in Cucumis melo (L.) using random amplified polymorphic DNA markers. Acta Horticulturae 461: 123-131, 1998

Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pine. Proceedings of the National Academy of Sciences of the United States of America 92(6): 2066-2070, 1995

Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus). Journal of Agricultural Science 133(4): 389-395, 1999

Use of random amplified polymorphic DNA markers for mapping the chickpea genome. Biologia Plantarum 44(2): 195-202, 2001

Genetic mapping of random amplified polymorphic DNA markers in Citrus. Hortscience 28(5): 222, 1993

A genetic linkage map of longleaf pine (Pinus palustris Mill.) based on random amplified polymorphic DNAs. Journal of Heredity 85(6): 433-439, 1994

Linkage of random amplified polymorphic DNA markers to downy mildew resistance in cucumber (Cucumis sativus L.). Euphytica 115(2): 105-113, 2000

Low levels of genetic diversity in red pine confirmed by random amplified polymorphic DNA markers. Canadian Journal of Forest Research 22(9): 1332-1337, 1992

Use of random amplified polymorphic DNA markers for gene mapping in common bean. Annual report of the Bean Improvement Cooperative 5(35): 62-63, 1992

Genomic mapping of Pinus sylvestris (L.) using random amplified polymorphic DNA markers. Forest Genetics 2(2): 109-116, 1995