EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Utility of random amplified polymorphic DNA markers for linkage mapping in Turkish red pine



Utility of random amplified polymorphic DNA markers for linkage mapping in Turkish red pine



Silvae Genetica 44(2-3): 110-116



We have applied the random amplified polymorphic DNA (RAPD) marker system to estimate linkage relationships in Pinus brutia TEN. We used DNA samples from 30 haploid seed megagametophytes from each of 4 mother trees. Ninety-five 10-base oligonucleotide primers were evaluated and 34 revealed at least one polymorphic RAPD locus. The number of segregating RAPD loci per polymorphic primer varied from 2.32 to 3.25, but when segregating loci per tested primer was considered it was low, ranging on the average, from 0.28 in tree 4 to 0.59 in tree 1. Based on the RAPD loci segregating in 1:1 ratio, genetic linkage groups formed from 6 for genotype-4 (total map distance=163.91 cM) to 13 for genotype-3 (total map distance= 511.2 cM). It was also found that a number of segregating loci in all 4 genotypes (ranging from 14 to 21) could not be assigned into any of the constructed linkage groups. It was difficult to compare linkage groups among the genotypes since most of RAPD loci segregating in one genotype were not found in others. Thus, the linkage map provided very little information on the genomic organization of RAPD markers at the species level. The utility of RAPD markers in forest genetics is also discussed in the paper.

(PDF emailed within 1 workday: $29.90)

Accession: 034168267

Download citation: RISBibTeXText



Related references

Utility of random amplified polymorphic DNA (RAPD) markers for linkage mapping in Turkish red pine (Pinus brutia Ten.). Silvae Genetica 44(2/3): 110-116, 1995

Comparison of homology and linkage of random amplified polymorphic DNA markers between individual trees of Scots pine. Molecular Ecology 8(1): 15-22, 1999

Comparison of homology and linkage of random amplified polymorphic DNA (RAPD) markers between individual trees of Scots pine (Pinus sylvestris L.). Molecular Ecology 8(1): 15-22, 1999

Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 89(5): 606-614, 1994

Intensive linkage mapping in a wasp and a mosquito with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers. Genetics 143(4): 1727-1738, 1996

Intensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers. Genetics 143(4): 1727-1738, 1996

Extensive linkage mapping in a wasp (Bracon hebetor) and a mosquito (Aedes aegypti) with single-strand conformation polymorphism analysis of random amplified polymorphic DNA markers. Genetics. August; 1434: 1727-1738, 1996

Construction of a linkage map in Cucumis melo (L.) using random amplified polymorphic DNA markers. Acta Horticulturae ( 461): 123-131, 1998

Linkage of random amplified polymorphic DNA markers in Pinus halepensis Mill. Silvae Genetica 51(5/6): 196-201, 2002

Random amplified polymorphic DNA markers tightly linked to a gene for resistance to white pine blister rust in sugar pine. Proceedings of the National Academy of Sciences of the United States of America 92(6): 2066-2070, 1995

Genetic mapping of random amplified polymorphic DNA markers in Citrus. Hortscience 28(5): 222, 1993

Use of random amplified polymorphic DNA markers for mapping the chickpea genome. Biologia Plantarum 44(2): 195-202, 2001

Linkage of random amplified polymorphic DNA, isozyme and morphological markers in grasspea (Lathyrus sativus). Journal of Agricultural Science 133(4): 389-395, 1999

Genomic mapping of Pinus sylvestris (L.) using random amplified polymorphic DNA markers. Forest Genetics 2(2): 109-116, 1995

Use of random amplified polymorphic DNA markers for gene mapping in common bean. Annual report of the Bean Improvement Cooperative5(35): 62-63, 1992