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A PCR-based assay for mating type determination in Magnaporthe grisea

Tredway, L.P.; Covert, S.F.; Gold, S.E.; Stevenson, K.L.; Burpee, L.L.

Phytopathology 91(6 Suppl): S203

2001


ISSN/ISBN: 0031-949X
Accession: 034279019

Magnaporthe grisea is a major pathogen of grasses, including several of economic importance. The genetic structure of M. grisea populations has important implications in disease management, and the distribution of mating types influences population structure. Our objective was to develop a PCR-based assay for determining mating type in M. grisea. Specific PCR primers were designed for each mating type idiomorph. The primer pairs specific to Mat1-1 and Mat1-2 mating types yielded products of 552 bp and 390 bp, respectively. The primers retained their specificity in multiplex PCR reactions, and the products were easily differentiated in a 1% agarose gel. The assay was tested on 40 M. grisea isolates of known mating type from several hosts. PCR products of the expected size were observed in all cases. Southern analysis was used to verify that the PCR products were homologous to the mating type idiomorphs. The assay was used to determine mating type distribution in Georgia populations of M. grisea. Preliminary results will be presented.

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