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Adhesion Molecules Expression on B-Cells Is Significantly Different among Peripheral Blood, Bone Marrow and Lymph Node Compartments, and Has Significant Interaction with Gender in Patients with B-Cell Chronic Lymphocytic Leukemia



Adhesion Molecules Expression on B-Cells Is Significantly Different among Peripheral Blood, Bone Marrow and Lymph Node Compartments, and Has Significant Interaction with Gender in Patients with B-Cell Chronic Lymphocytic Leukemia



Blood 100(11): Abstract No. 2347



Patients with B-CLL have variable tumor mass distribution within major cell compartments, and as a consequence different clinical presentation. Distribution pattern can be assessed clinically, has prognostic impact and has unexpected gender interactions (Jaksic O et al, Haematologica, 2001;86:827-836). To evaluate possible role of adhesion molecules expressed on tumor cells homing, we analyzed a panel including CD11a, CD11b, CD11c, CD18, CD29, CD31, CD44, CD44v4, CD49c, CD49d, CD50, CD54, CD61, CD62L and CD102 in peripheral blood (PB), bone marrow (BM) and lymph nodes (LN) in 27 B-cell CLL patients with typical immunophenotype (CD5/CD19 co-expression, CD23+, and dim light chain expression). Samples were taken on the same day from PB and BM by conventional techniques and by thin needle aspirate from LN compartment. Cells were processed by ficoll-gradient and mononuclear cells were analyzed by flow-cytometry. Mean Fluorescence Intensity (MFI) of adhesion molecules on CD19+ cells was compared among compartments in each patient by paired t-test. Three typical MFI expression patterns have been observed: 1. LNBM>PB; 3. LN>PB>BM. Analysis on overall patient population sowed significant difference of type 1. for CD102 (p=0.0010); of type 2. for CD54 (p=0.0052) and CD44v4 (p=0.0019), and of type 3. for CD18 (p=0.012), CD11a (p=0.017) and CD11c (p=0.022), while other analyzed adhesion molecules did not show significant difference among compartments. Analysis by gender subsets disclosed additional interesting interactions: for CD44 of type 1. (p=0.024); for CD50 of type 2. (p=0.032) and for CD62L (p=0.020) and CD11b (p=0.034) of type 3. differences were found exclusively in males. In contrast, type 3. difference was found for CD38 (p=0.011) exclusively in females. This study shows that expression of selected adhesion molecules on tumor cells is significantly different among tumor cell compartments, indicating their possible role in resulting clinical presentation. The role of gender in homing of tumor cells, observed previously on clinical grounds, disclosed significantly different behavior for adhesion molecules as well. Taking together, adopted investigation approach proved feasible, disclosed yet unexplained interesting interactions and warrants further studies.

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