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Amylase activities and carbohydrate contents in stems and roots of phasey bean Urb after defoliation


Amylase activities and carbohydrate contents in stems and roots of phasey bean Urb after defoliation



Grassland Science 49(1): 16-22



ISSN/ISBN: 1744-6961

Our objective in this experiment was to research the relationships among regrowth rates, amylase activities and storage carbohydrates after defoliation as affected by growth temperature in phasey bean (Macroptilium lathyroides (L.) Urb.). Plants were grown in the controlled environmental chamber set at 25degreeC prior to defoliation. The defoliated plants were regrown in those chambers of 20, 25 or 30degreeC. New shoot production, amylase activities and carbohydrate contents in stems and roots were measured on days 0, 1, 3 and 6 after defoliation. New shoot production developed remarkably faster with increasing growth temperature. At day 3 and 6, starch contents in stems were lower in the plants highly producing new shoots. Glucose contents were higher in stems than in roots and sucrose contents were higher in roots than in stems just before defoliation, and thereafter there were rapid decreases in glucose contents for stems and in sucrose contents for both organs. Total amylase activities did not change during the regrowth period, but alpha-amylase activity was positively correlated with either growth temperature or regrowth days in both organs. As the concomitant decrease in starch contents with the increase in alpha-amylase activities was observed only on day 6 in the stems with increasing growth temperature.

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Accession: 034386721

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