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CDNA microarray analysis to identify genes preferentially expressed during appressorium formation in the rice blast fungus, Magnaporthe grisea

CDNA microarray analysis to identify genes preferentially expressed during appressorium formation in the rice blast fungus, Magnaporthe grisea

Phytopathology 91(6 Suppl): S87

ISSN/ISBN: 0031-949X

Formation of a functional appressorium is essential for penetration into host plant by rice blast fungus, Magnaporthe grisea. So far, relatively few genes have been identified through studies of limited scope and shown to be involved in the appressorium formation. In the present study, a high density cDNA microarray was employed to examine the expression profile of a large number of genes associated with appressorium formation. A microarray containing 4582 cDNAs derived from cDNA libraries of the appressorium formation stage and the vegetative stage was constructed. This array is being used to identify a suite of genes that are differentially expressed during appressorium formation through hybridization against mRNA probes obtained from different developmental stages and from appressorium-defective mutants. A detailed expression profiling of appressorium formation-related genes will be presented.

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Accession: 034515494

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