+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Calcium-induced calcium release is protected from the damaging effects of fatiguing stimulation in slow- but not in fast-twitch skeletal muscles

Calcium-induced calcium release is protected from the damaging effects of fatiguing stimulation in slow- but not in fast-twitch skeletal muscles

FASEB Journal 16(5): A775

Damaging effects of fatigue are caused by the disruption of the normal excitation-contraction coupling process; calcium uptake and/or calcium release by the sarcoplasmic reticulum. By using both intact and single skinned muscle fibers, we report here for the first time that fatiguing stimulation abolishes CICR in EDL but not in soleus (SOL). Fatiguing stimulation did not affect the contractile proteins or calcium-uptake by the SR of fatigued skinned fibers from either EDL or SOL muscles. Loss of CICR in skinned muscle fibers was reflected in intact EDL muscles, since caffeine, an agent known to enhance CICR, promptly restored contractile function in fatigued EDL muscles at the end of a 30 min recovery period. In contrast, caffeine had no additional effects on the contractile function of intact fatigued SOL muscles at the end of the recovery period since they had already achieved full recovery at that time. We hypothesize that fatiguing stimulation causes oxidative modifications of the ryanodine receptor and/or its accessory proteins leading to the disruption of CICR in EDL muscles. We speculate that this does not occur in SOL muscles because of their higher intracellular levels of antioxidants.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 034522095

Download citation: RISBibTeXText

Related references

Differential effects of acidosis on calcium activated force and atpase of fast twitch and slow twitch skeletal muscles of the rabbit. Journal of Physiology (Cambridge) 390: 152P, 1987

The role of parvalbumin in the decay of calcium after electrical stimulation in intact rat fast-twitch and slow-twitch skeletal muscle fibers. FASEB Journal 10(6): A1395, 1996

Comparison of calcium release from sarcoplasmic reticulum of slow and fast twitch muscles. Journal of Membrane Biology 122(2): 155-164, 1991

Characterization of fast-twitch and slow-twitch skeletal muscles of calsequestrin 2 (CASQ2)-knock out mice: unexpected adaptive changes of fast-twitch muscles only. Journal of Muscle Research and Cell Motility 37(6): 225-233, 2017

Resolving the Calcium Release Machinery of Mammalian Fast- and Slow-Twitch Skeletal Muscle. Biophysical Journal 106(2): 123a-124a, 2014

Decay of calcium transients after electrical stimulation in rat fast- and slow-twitch skeletal muscle fibres. Journal Of Physiology (cambridge). 501(3): 573-588, 1997

Caffeine induced free calcium changes in intact fast- and slow-twitch muscles. FASEB Journal 12(5): A1030, 1998

The effects of fatiguing stimulation on calcium-induced calcium release of skinned muscle fibers. Biophysical Journal 80(1 Part 2): 379a-380a, 2001

Observation of the molecular organization of calcium release sites in fast- and slow-twitch skeletal muscle with nanoscale imaging. Journal of the Royal Society, Interface 11(99), 2015

Mechanism of calcium release from sarcoplasmic reticulum sr of slow and fast twitch skeletal muscle ryanodine binding studies. Biophysical Journal 55(2 Part 2): 97A, 1989

Effect of de nervation and tenotomy on the calcium transport of sarcoplasmic reticulum of fast twitch and slow twitch muscles. Acta Physiologica Academiae Scientiarum Hungaricae 56(1): 35, 1980

Sarcoplasmic reticulum calcium pump in cardiac and slow twitch skeletal muscle but not fast twitch skeletal muscle undergoes phosphorylation by endogenous and exogenous Ca2+/calmodulin-dependent protein kinase. Characterization of optimal conditions for calcium pump phosphorylation. Journal of Biological Chemistry 269(49): 31198-31206, 1994

Force-velocity characteristics for calcium-activated mammalian slow-twitch and fast-twitch skeletal fibers from the guinea pig. Proceedings of the National Academy of Sciences of the United States of America 73(12): 4693-4697, 1976

Calcium transients and calcium release in rat fast-twitch skeletal muscle fibres. Journal of Physiology 463: 709-728, 1993

Phospholamban expressed in slow-twitch and chronically stimulated fast-twitch muscles minimally affects calcium affinity of sarcoplasmic reticulum Ca(2+)-ATPase. Journal of Biological Chemistry 267(36): 26056-26061, 1992