+ Site Statistics
References:
54,258,434
Abstracts:
29,560,870
PMIDs:
28,072,757
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Characterisation of Toxoplasma gondii isolates using polymerase chain reaction and restriction fragment length polymorphism of the non-coding Toxoplasma gondii -gene sequences



Characterisation of Toxoplasma gondii isolates using polymerase chain reaction and restriction fragment length polymorphism of the non-coding Toxoplasma gondii -gene sequences



International Journal for Parasitology 30(7): 853-858



The Toxoplasma gondii (TGR) genes constitute a family of non-coding sequences, three of which have been previously described as possible tools for typing of Toxoplasma gondii isolates. We obtained new isolates of T. gondii from domestic and wild animals, and used these to evaluate the possibility of using TGR gene variants as markers to distinguish among T. gondii isolates from different animals and different geographical sources. Based on the band patterns obtained by restriction fragment length polymorphism (RFLP) analysis of the polymerase chain reaction (PCR) amplified TGR sequences, the T. gondii isolates could be separated into seven groups. Sequencing the amplified products showed that at least 20 TGR sequences not hitherto described had been found, demonstrating that the TGR gene family comprises a large number of different yet highly homologous sequences. Each isolate had its own unique TGR sequence. The TGR gene family therefore seems a promising target for typing individual T. gondii isolates and for studying the genetic distance between two isolates, which can be used for tracing routes of infection.

(PDF emailed within 1 workday: $29.90)

Accession: 034554940

Download citation: RISBibTeXText


Related references

Characterisation of Toxoplasma gondii isolates using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) of the non-coding Toxoplasma gondii (TGR)-gene sequences. International Journal for Parasitology. June; 307: 853-858, 2000

Generation of a restriction fragment length polymorphism linkage map for Toxoplasma gondii. Genetics 132(4): 1003-1015, 1992

Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR-restriction fragment length polymorphism analysis at the B1 gene. Journal of Clinical Microbiology 39(1): 398-400, 2001

Restriction fragment length polymorphism and virulence of Czech Toxoplasma gondii strains. International Journal for Parasitology 28(9): 1367-1374, 1998

Amplification of the hsp70 genes among different geographical strains of isolates of Toxoplasma gondii and its restriction fragment length analysis. Chinese Journal of Zoonoses. May; 203: 199-202, 2004

Direct detection of Toxoplasma gondii with polymerase chain reaction in diagnosis of fetal toxoplasma infection. Wiener Klinische Wochenschrift 107(4): 137-140, 1995

Polymerase chain reaction for Toxoplasma gondii DNA detection in the cerebrospinal fluid of AIDS patients with acute Toxoplasma encephalitis. IXTH INTERNATIONAL CONFERENCE ON AIDS AND THE IVTH STD WORLD CONGRESS [Author] IXth International Conference on AIDS in affiliation with the IVth STD World Congress : 386, 1993

Role of Toxoplasma gondii HSP70 and Toxoplasma gondii HSP30/bag1 in antibody formation and prophylactic immunity in mice experimentally infected with Toxoplasma gondii. Microbiology and Immunology 43(5): 471-479, 1999

Restriction Fragment Length Polymorphism-based Genotyping of Toxoplasma gondii from Autopsy-Proven Cases of Acquired Immunodeficiency Syndrome-associated Cerebral Toxoplasmosis. Annals of Indian Academy of Neurology 21(4): 250-255, 2018

Detection of Toxoplasma gondii Parasitemia by Culture and Polymerase Chain Reaction in AIDS Patients with Suspected Toxoplasma Encephalitis A Prospective Study. AMERICAN SOCIETY FOR MICROBIOLOGY Human retroviruses and related infections : 110, 1995

Characterisation of the lpdA gene from Neisseria meningitidis by polymerase chain reaction, restriction fragment length polymorphism and sequencing. FEMS Microbiology Letters 174(1): 191-199, May 1, 1999

Detection of the pathogenic parasite Toxoplasma gondii by specific amplification of ribosomal sequences using comultiplex polymerase chain reaction. Journal of Clinical Microbiology 31(2): 203-207, 1993

Restriction-fragment-length polymorphisms among Toxoplasma gondii strains. Parasitology Research 77(3): 266-268, 1991

Diagnostic Value of Nested Polymerase Chain Reaction for Detection of Toxoplasma Gondii in Aqueous Humor and Peripheral Blood Specimens of Clinically Suspected Cases of Toxoplasma Chorioretinitis. ARVO Annual Meeting Abstract Search & Program Planner : Abstract No 4299, 2002

Studies on the gene probe amplified and labeled by polymerase chain reaction (PCR) in detection of Toxoplasma gondii. Chinese Journal of Zoonoses 14(3): 36-39, 1998