+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Characterization of a chemosensory protein from honeybee as a brood pheromone carrier



Characterization of a chemosensory protein from honeybee as a brood pheromone carrier



European Journal of Biochemistry 269(18): 4586-4596



Chemosensory proteins (CSPs) are ubiquitous soluble small proteins isolated from sensory organs of a wide range of insect species, which are believed to be involved in chemical communication. We report the cloning of a honeybee CSP gene called ASP3c, as well as the structural and functional characterization of the encoded protein. The protein was heterologously secreted by the yeast Pichia pastoris using the native signal peptide. ASP3c disulfide bonds were assigned after trypsinolysis followed by chromatography and mass spectrometry combined with microsequencing. The pairing (Cys(I)-Cys(II), Cys(III)-Cys(IV)) was found to be identical to that of Schistocerca gregaria CSPs, suggesting that this pattern occurs commonly throughout the insect CSPs. CD measurements revealed that ASP3c mainly consists of alpha-helices, like other insect CSPs. Gel filtration analysis showed that ASP3c is monomeric at neutral pH. Using ASA, a fluorescent fatty acid anthroyloxy analogue as a probe, ASP3c was shown to bind specifically to large fatty acids and ester derivatives, which are brood pheromone components, in the micromolar range. It was unable to bind tested general odorants and other tested pheromones (sexual and nonsexual). This is the first report on a natural pheromonal ligand bound by a recombinant CSP with a measured affinity constant.

(PDF emailed within 1 workday: $29.90)

Accession: 034558976

Download citation: RISBibTeXText


Related references

1H, 13C and 15N chemical shift assignment of the honeybee pheromone carrier protein ASP1. Journal of Biomolecular Nmr 20(2): 183-184, 2001

Effect of a brood pheromone on honeybee hypopharyngeal glands. Comptes Rendus de l'Academie des Sciences. Serie Iii, Sciences de la Vie 319(9): 769-772, 1996

Primer effects of a brood pheromone on honeybee behavioural development. Proceedings. Biological Sciences 268(1463): 163-168, 2001

Characterization of a brood pheromone isolated from the sexual brood of the imported fire ant, Solenopsis invicta. Annals of the Entomological Society of America 68(2): 301-304, 1975

Brood pheromone modulates honeybee (Apis mellifera L.) sucrose response thresholds. Behavioral Ecology and Sociobiology 49(2/3): 206-213, 2001

Potential of Honeybee Brood Pheromone to Enhance Foraging and Yield in Hybrid Carrot Seed. Horttechnology 25(1): 98-104, 2015

Molecular binding characterization with chemical ligands of a chemosensory protein AcerCSP3 in the Chinese honeybee, Apis cerana cerana Fabricius Hymenoptera Apidae. Acta Entomologica Sinica 54.3 (March 20): 259-264, 2011

Molecular cloning, characterization, and expression pattern of chemosensory protein 1 gene Acer-CSP1 in the Chinese honeybee, Apis cerana cerana Hymenoptera Apidae. Acta Entomologica Sinica 53.9 (September 20): 962-968, 2010

Chemosensory proteins of the eastern honeybee, Apis cerana: Identification, tissue distribution and olfactory related functional characterization. Comparative Biochemistry and Physiology. Part B, Biochemistry and Molecular Biology 194-195: 11-19, 2016

Effect of type of brood comb on chalk brood disease in honeybee colonies. Journal of Apicultural Research, 251: 58-62, 1986

Identification of odorant binding proteins and chemosensory proteins in Microplitis mediator as well as functional characterization of chemosensory protein 3. Plos One 12(7): E0180775, 2017

Morphological elements and proteins in the haemolymph of honeybee brood infected with American foul brood. Annales Universitatis Mariae Curie Sklodowska, DD, Medicina Veterinaria 26(11): 119-130, 1971

Regulation of protein balance in the honeybee colony by brood cannibalism. Apidologie 15(3): 339-353, 1984

Digestion of protein and brood care in the honeybee (Apis mellifera L.). Verhandlungen der Deutschen Zoologischen Gesellschaft, 81: 226-227, 1988

Effect of type of brood comb on chalk brood disease in honeybee apis mellifera colonies. Journal of Apicultural Research 25(1): 58-62, 1986