Cloning and construction of plant expression vector of dihydroflavonol reductase gene and transgenic Nicotiana tabacum
WangYanXiu; ZhangJinWen; WuLuGuang
Acta Botanica Boreali Occidentalia Sinica: 1, 1-7
The dihydroflavonol reductase (DFR) gene was cloned from young fruit of Medicago truncatula 5160 by RT-PCR and the coding sequences of this gene were analyzed. The result indicated that the full-length cDNA is 1 018 bp and contain open reading frame (ORF) encoding 337 amino-acids. The sequences were analyzed with software Blast and exhibited homologous 99.80% with DFR from GenBank.