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Cloning putative parasitism genes expressed in the esophageal glands of Meloidogyne incognita

Cloning putative parasitism genes expressed in the esophageal glands of Meloidogyne incognita

Phytopathology 92(6 Suppl): S37

ISSN/ISBN: 0031-949X

Cloning parasitism genes coding proteins secreted from the esophageal glands and injected through the stylet into plant tissue is the key to understanding the molecular basis of nematode parasitism of plants. Microaspiration of cytoplasm from the glands of 43 parasitic stages of M. incognita provided mRNA for RT-PCR to construct a long-distance cDNA library. Of 1,200 cDNAs sequenced, deduced protein sequences of 141 cDNAs were preceded by a secretion signal peptide and, thus, may function in nematode parasitism of plants. In mRNA in situ hybridizations, 21 cDNAs coding signal peptides specifically hybridized with mRNA within the subventral (7) or dorsal (14) glands. PSORT II predicted 18 deduced proteins to be extracellular and 3 as nuclear localized. In BLASTp analyses, 19 predicted proteins were novel and two had similarities to a transcription factor and an avirulence protein. None of the gland-expressed genes had homologues in Caenorhabditis elegans.

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Accession: 034596504

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