+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Coagulation, inflammation, and pregnancy complications

Coagulation, inflammation, and pregnancy complications

Lancet 363(9403): 96-97

In this study, the kinetic parameters, V(max) and K(m), of rat liver DT-diaphorase were determined for a series of p-benzoquinones, with methyl, methoxy, cyano, hydroxy and halo substituents. The results show that there is no correlation between the experimentally determined rates of p-benzoquinone reduction by DT-diaphorase and the calculated chemical reactivity of the examined substrates as expressed by the energy of the lowest unoccupied molecular orbital, E(LUMO). However, a reasonable correlation was found between the natural logarithm of V(max)/K(m) and the partition coefficient of the p-benzoquinones (r=0.81). Furthermore, tetrachloro-p-benzoquinone, one of the tested quinones is shown to be an inhibitor of rat DT-diaphorase. The presence of bovine serum albumin (BSA) in the incubation mixture protects DT-diaphorase against the inactivation by tetrachloro-p-benzoquinone, probably by interacting with the quinone. Maldi-Tof analysis of the incubation mixture of the purified DT-diaphorase and tetrachloro-p-benzoquinone showed that every subunit of the enzyme shifted about +414 amu, whereas the dimer shifted about +849 amu relative to control values. This indicates a covalent modification of the rat liver DT-diaphorase by tetrachloro-p-benzoquinone.

(PDF emailed within 0-6 h: $19.90)

Accession: 034599108

Download citation: RISBibTeXText

PMID: 14726160

DOI: 10.1016/S0140-6736(03)15309-3

Related references

Role of tissue factor in pregnancy complications: crosstalk between coagulation and inflammation. Thrombosis Research 127 Suppl 3: S43-S46, 2011

Coagulation status and complications of pregnancy. Thrombosis Research 115(6): 461-467, 2005

Recurrent pregnancy loss: a disease of inflammation and coagulation. Journal of Obstetrics and Gynaecology Research 35(4): 609-622, 2009

Significance of intravascular blood coagulation in the pathogenesis of pregnancy complications. Akusherstvo i Ginekologiia 1977(3): 1-4, 1977

More fibrosis and thrombotic complications but similar expression patterns of markers for coagulation and inflammation in symptomatic plaques from DM2 patients. Journal of Histochemistry and Cytochemistry 52(9): 1141-1149, 2004

Sterile inflammation and pregnancy complications: a review. Reproduction 152(6): R277-R292, 2016

Soluble fibrin in plasma as a sign of activated coagulation in patients with pregnancy complications. Acta Obstetricia et Gynecologica Scandinavica 77(2): 165-169, 1998

Possibility of Coagulation System Activation Determination with Tissue Factor in Pregnancy Complications. Clinical Laboratory 62(10): 1851-1856, 2017

Aberrant maternal inflammation as a cause of pregnancy complications: A potential therapeutic target?. Placenta 36(8): 960-966, 2016

Cytokine and cytokine-like inflammation markers, endothelial dysfunction, and imbalanced coagulation in development of diabetes and its complications. Journal of Clinical Endocrinology and Metabolism 94(9): 3171-3182, 2009

Pregnancy complications associated with antiphospholipid antibodies and systemic lupus erythematosus: disorders of inflammation. Journal of Reproductive Immunology 90(2): 153-0, 2011

Inflammation in pregnancy: its roles in reproductive physiology, obstetrical complications, and fetal injury. Nutrition Reviews 65(Supplement s3): 0-0, 2007

Inflammation in pregnancy: its roles in reproductive physiology, obstetrical complications, and fetal injury. Nutrition Reviews 65(12 Pt 2): S194-S202, 2008

Inflammation and Thrombophilia in Pregnancy Complications: Implications for Risk Assessment and Clinical Management. Cardiovascular and Hematological Disorders Drug Targets 15(3): 187-203, 2016

Nanosilica-induced placental inflammation and pregnancy complications: Different roles of the inflammasome components NLRP3 and ASC. Nanotoxicology 9(5): 554-567, 2016