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Detection of cagA gene and typing of vacA gene in Helicobacter pylori strains isolated in the Czech Republic



Detection of cagA gene and typing of vacA gene in Helicobacter pylori strains isolated in the Czech Republic



Abstracts of the General Meeting of the American Society for Microbiology 103: D-071



Helicobacter pylori infection is main factor inducing chronic gastritis. Eventhough infection is often asymptomatic, approx. 10% infected individuals develope gastric and/or duodenal ulcer, carcinoma or MALT lymphoma. Molecular methods enable analysis of genes encoding bacteria's virulence factors. Some results obtained in different countries were already published, but no study providing data from czech population exists. We have analysed 69 Helicobacter strains isolated from patients of different endoscopic diagnosis (bulbitis-BU 7(10.1%), gastritis-GA 9(3%) gastrobulbitis-GA+BU 6(9%), duodenal ulcer-DU 25(36%),gastric ulcer-GU 12 (17%)),using DNA amplification by PCR technique with digoxigeninated nucleotides followed by capture-probe hybridization and ELISA detection of the product. Specific probe was used for detection of cagA gene and 5 different specific probes for signal peptide subtypes sequences sla, sib and s2 and alelic ml and m2 types were used for vacA gene typing. Diagnostic test has been adapted for routine use, benefiting of a stripe microwell formate. Out of a total of 69 strains, 46 (67%) were cag A positive. Typing of the vacA genes provided following data: vacAs1: 42(61%),vacA s2: 11(16%), vacA ml: 26(38%), vacA m2: 41(59%). Highest incidence of cagA+ strains was found in patients with duodenal ulcer and bulbitis followed by gastric ulcer. No significant difference in endoscopic diagnosis among types of vacAs and vacAm, except high frequence of vacAm2 type (91.7%) in gastric ulcer patients group, has been observed.

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