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Determination of polycyclic aromatic hydrocarbons in shellfish and fish by gel permeation chromatography and high performance liquid chromatography


Determination of polycyclic aromatic hydrocarbons in shellfish and fish by gel permeation chromatography and high performance liquid chromatography



Alimentaria 40(Separata Al Numero 340): 3-14



This paper describes an appropriate analytical method to determine the contents of polycyclic aromatic hydrocarbons in samples of shellfish and fish by high performance liquid chromatography with fluorescence detection including a previous step to purify the crude extracts by gel permeation chromatography. This method is based and enlarges the scope of a procedure described formerly for the specific determination of polycyclic aromatic hydrocarbons in pomace olive oil (Alimentaria, 328, 117-126, 2001). The method has been internally validated for the determination of the eight polycyclic aromatic hydrocarbons specified in the Orden of July 25, 2001, published in the Spanish Official State Bulletin no. 178: benzo(a)pyrene. benzo(e)pyrene. benzo(a)anthracene. benzo(b)fluoranthene, benzo(k)fluoranthene, dibenzo(a,h)anthracene, benzo(g,h,i)perylene and indeno(1,2,3-c,d)pyrene, in fresh mussels and horse mackerel. The limit of determination was 0.5 pg/kg for each hydrocarbon.

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Accession: 034710216

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