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Development of rice cultivars with improved blast resistance using marker assisted selection



Development of rice cultivars with improved blast resistance using marker assisted selection



Phytopathology 93(6 Supplement): S41, June



Marker assisted selection is being employed in rice breeding to accelerate the development of cultivars with improved rice blast Magnaporthe grisea resistance. A dominant gene marker was used to monitor the incorporation of the rice blast resistance gene Pi-ta in 2,600 genomic DNA samples of F3 families and advanced breeding lines. PCR using primers specifically amplifying a resistant allele of the Pi-ta gene was analyzed by agarose gel electrophoresis. In one group of 2,109 samples of 7 3F3 populations, about 33% were homozygous for the resistant allele, 17% were homozygous for the susceptible allele, and 50% were heterozygous as determined by the Pi-ta marker. This marker was used to verify the results of pathogenicity tests. When seedlings were inoculated with isolates containing the corresponding avirulence gene AVR-Pi-ta, those seedlings determined to have the resistant allele were resistant to infection. Seedlings determined to have the susceptible allele were susceptible to infection. Use of Pi-ta marker data increases the efficiency and speed of cultivar development.

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Accession: 034717956

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