+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Development of rice cultivars with improved blast resistance using marker assisted selection



Development of rice cultivars with improved blast resistance using marker assisted selection



Phytopathology 93(6 Supplement): S41



Marker assisted selection is being employed in rice breeding to accelerate the development of cultivars with improved rice blast Magnaporthe grisea resistance. A dominant gene marker was used to monitor the incorporation of the rice blast resistance gene Pi-ta in 2,600 genomic DNA samples of F3 families and advanced breeding lines. PCR using primers specifically amplifying a resistant allele of the Pi-ta gene was analyzed by agarose gel electrophoresis. In one group of 2,109 samples of 7 3F3 populations, about 33% were homozygous for the resistant allele, 17% were homozygous for the susceptible allele, and 50% were heterozygous as determined by the Pi-ta marker. This marker was used to verify the results of pathogenicity tests. When seedlings were inoculated with isolates containing the corresponding avirulence gene AVR-Pi-ta, those seedlings determined to have the resistant allele were resistant to infection. Seedlings determined to have the susceptible allele were susceptible to infection. Use of Pi-ta marker data increases the efficiency and speed of cultivar development.

(PDF emailed within 1 workday: $29.90)

Accession: 034717956

Download citation: RISBibTeXText


Related references

Pyramiding three genes with resistance to blast by marker-assisted selection to improve rice blast resistance of Jin 23B. Chinese Journal Of Rice Science: 1, 23-27, 2008

Pyramiding three genes with resistance to blast by marker-assisted selection to improve rice blast resistance of jin 23B. Zhongguo Shuidao Kexue 22(1): 23-27, 2008

Pyramiding three genes with resistance to blast by marker-assisted selection to improve rice blast resistance of Jin 23B. Chinese Journal Of Rice Science: 1, 23-27, 2008

Introgression of the rice blast resistance genes Pi1, Pi2 and Pi33 into Russian rice varieties by marker-assisted selection. Indian Journal of Genetics and Plant Breeding 76(1): 18-23, 2016

Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight. Field Crops Research 184: 1-8, 2015

Marker-assisted selection and pyramiding for three blast resistance genes, Pi-d(t)1, Pi-b, Pi-ta2, in rice. Sheng Wu Gong Cheng Xue Bao 20(5): 708-714, 2005

Marker-assisted selection for the rice blast resistance gene Pi-ar in a backcross population. Crop Breeding and Applied Biotechnology 10(1): 23-31, 2010

SSRs for Marker-Assisted Selection for Blast Resistance in Rice Oryza sativa L. Plant Molecular Biology Reporter 30(1): 79-86, 2012

Factors affecting the expression of durable resistance of rice cultivars to blast caused by Pyricularia grisea Sacc. 1. Selection of durably resistant cultivars for rice blast. Korean Journal of Plant Pathology 12(2): 231-236, 1996

Molecular markers linked to the blast resistance gene Pi-z in rice for use in marker-assisted selection. Tag. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik 107(6): 1014-1020, 2003

Molecular marker-assisted selection in a recurrent backcross breeding for the incorporation of resistance to rice stripe virus and panicle blast in rice (Oryza sativa L.). 2004

Improvement of rice blast resistance in TGMS line by pyramiding of Pi-1 and Pi-2 through molecular marker-assisted selection. 2008

Phenotypic screening and molecular analysis of blast resistance in fragrant rice for marker assisted selection. Comptes Rendus Biologies 337(5): 318-324, 2015

Introgression of Blast Resistance Genes (Putative Pi-b and Pi-kh) into Elite Rice Cultivar MR219 through Marker-Assisted Selection. Frontiers in Plant Science 6: 1002, 2016

Breeding of rice restorer lines carrying blast resistance gene Pi25 by marker-assisted selection. Chinese Journal Of Rice Science: 6, 590-596, 2008